James then found a position at UMASS Lowell, where he founded one of the world’s first Centers for Bioinformatics. He helped develop that institutions’ undergraduate and graduate studies curriculum in Bioinformatics. He routinely had 20 or more students performing analyses for research projects, asking questions about the impact of algorithm selection on the biological inferences being made in many types of diseases. He produced online software that implemented numerous methods of microarray data analysis – and that provided objective performance evaluation measures to aid in the selection of the optimal methods. He studied gene expression in cancer, publishing a paper on overcoming experimental design flaws such as confounded controls. He taught courses in biology, genetics, and bioinformatics, and set up a sequencing lab for the Department of Biological Sciences.
But both Rolling Stone and The Skeptical Raptor view James Lyons-Weiler as an anti-vaxxer, so he might not be too conversant about medical matters either.
Since he is an anti-vaxxer, any medical opinion Lyons-Weiler utters will require quadruple verification from non-vaxxer scientists before I believe it.
It is interesting that his theory about the origins coronavirus portrays vaccine makers as villains. It would make a good plot for an end of the world movie, but as science there is a 99.999 percent chance it is utter crap.
Download citation Share Download full-text PDF The possible origins of 2019-nCoV coronavirus Preprint (PDF Available) · February 2020 with 547 Reads DOI: 10.13140/RG.2.2.21799.29601 Cite this publicationBotao Xiao21.93South China University of TechnologyLei Xiao Abstract The 2019-nCoV has caused an epidemic of 28,060 laboratory-confirmed infections in human including 564 deaths in China by February 6, 2020. Two descriptions of the virus published on Nature this week indicated that the genome sequences from patients were almost identical to the Bat CoV ZC45 coronavirus. It was critical to study where the pathogen came from and how it passed onto human. An article published on The Lancet reported that 27 of 41 infected patients were found to have contact with the Huanan Seafood Market in Wuhan. We noted two laboratories conducting research on bat coronavirus in Wuhan, one of which was only 280 meters from the seafood market. We briefly examined the histories of the laboratories and proposed that the coronavirus probably originated from a laboratory. Our proposal provided an alternative origin of the coronavirus in addition to natural recombination and intermediate host. Discover the world's research 15+ million members 118+ million publications 700k+ research projects Join for free
Content uploaded by Botao Xiao Author content Content may be subject to copyright. Download full-text PDF The possible origins of 2019-nCoV coronavirus Botao Xiao1,2* and Lei Xiao3 1 Joint International Research Laboratory of Synthetic Biology and Medicine, School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China 2 School of Physics, Huazhong University of Science and Technology, Wuhan 430074, China 3 Tian You Hospital, Wuhan University of Science and Technology, Wuhan 430064, China * Corresponding author: [email protected] Tel / Fax: 86-20-3938-0631 The 2019-nCoV coronavirus has caused an epidemic of 28,060 laboratory-confirmed infections in human including 564 deaths in China by February 6, 2020. Two descriptions of the virus published on Nature this week indicated that the genome sequences from patients were 96% or 89% identical to the Bat CoV ZC45 coronavirus originally found in Rhinolophus affinis 1,2. It was critical to study where the pathogen came from and how it passed onto human. An article published on The Lancet reported that 41 people in Wuhan were found to have the acute respiratory syndrome and 27 of them had contact with Huanan Seafood Market 3. The 2019-nCoV was found in 33 out of 585 samples collected in the market after the outbreak. The market was suspicious to be the origin of the epidemic, and was shut down according to the rule of quarantine the source during an epidemic. The bats carrying CoV ZC45 were originally found in Yunnan or Zhejiang province, both of which were more than 900 kilometers away from the seafood market. Bats were normally found to live in caves and trees. But the seafood market is in a densely-populated district of Wuhan, a metropolitan of ~15 million people. The probability was very low for the bats to fly to the market. According to municipal reports and the testimonies of 31 residents and 28 visitors, the bat was never a food source in the city, and no bat was traded in the market. There was possible natural recombination or intermediate host of the coronavirus, yet little proof has been reported. Was there any other possible pathway? We screened the area around the seafood market and identified two laboratories conducting research on bat coronavirus. Within ~280 meters from the market, there was the Wuhan Center for Disease Control & Prevention (WHCDC) (Figure 1, from Baidu and Google maps). WHCDC hosted animals in laboratories for research purpose, one of which was specialized in pathogens collection and identification 4- 6. In one of their studies, 155 bats including Rhinolophus affinis were captured in Hubei province, and other 450 bats were captured in Zhejiang province 4. The expert in collection was noted in the Author Contributions (JHT). Moreover, he was broadcasted for collecting viruses on nation-wide newspapers and websites in 2017 and 2019 7,8. He described that he was once by attacked by bats and the blood of a bat shot on his skin. He knew the extreme danger of the infection so he quarantined himself for 14 days 7. In another accident, he quarantined himself again because bats peed on him. He was once thrilled for capturing a bat carrying a live tick 8. Surgery was performed on the caged animals and the tissue samples were collected for DNA and RNA extraction and sequencing 4, 5. The tissue samples and contaminated trashes were source of pathogens. They were only ~280 meters from the seafood market. The WHCDC was also adjacent to the Union Hospital (Figure 1, bottom) where the first group of doctors were infected during this epidemic. It is plausible that the virus leaked around and some of them contaminated the initial patients in this epidemic, though solid proofs are needed in future study. The second laboratory was ~12 kilometers from the seafood market and belonged to Wuhan Institute of Virology, Chinese Academy of Sciences 1, 9, 10. This laboratory reported that the Chinese horseshoe bats were natural reservoirs for the severe acute respiratory syndrome coronavirus (SARS-CoV) which caused the 2002-3 pandemic 9. The principle investigator participated in a project which generated a chimeric virus using the SARS-CoV reverse genetics system, and reported the potential for human emergence 10. A direct speculation was that SARS-CoV or its derivative might leak from the laboratory. In summary, somebody was entangled with the evolution of 2019-nCoV coronavirus. In addition to origins of natural recombination and intermediate host, the killer coronavirus probably originated from a laboratory in Wuhan. Safety level may need to be reinforced in high risk biohazardous laboratories. Regulations may be taken to relocate these laboratories far away from city center and other densely populated places. Contributors BX designed the comment and performed literature search. All authors performed data acquisition and analysis, collected documents, draw the figure, and wrote the papers. Acknowledgements This work is supported by the National Natural Science Foundation of China (11772133, 11372116). Declaration of interests All authors declare no competing interests. References 1. Zhou P, Yang X-L, Wang X-G, et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature 2020. https://doi.org/10.1038/s41586-020-2012-7. 2. Wu F, Zhao S, Yu B, et al. A new coronavirus associated with human respiratory disease in China. Nature 2020. https://doi.org/10.1038/s41586-020-2008-3. 3. Huang C, Wang Y, Li X, et al. Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. The Lancet 2019. https://doi.org/10.1016/S0140- 6736(20)30183-5. 4. Guo WP, Lin XD, Wang W, et al. Phylogeny and origins of hantaviruses harbored by bats, insectivores, and rodents. PLoS pathogens 2013; 9(2): e1003159. 5. Lu M, Tian JH, Yu B, Guo WP, Holmes EC, Zhang YZ. Extensive diversity of rickettsiales bacteria in ticks from Wuhan, China. Ticks and tick-borne diseases 2017; 8(4): 574-80. 6. Shi M, Lin XD, Chen X, et al. The evolutionary history of vertebrate RNA viruses. Nature 2018; 556(7700): 197-202. 7. Tao P. Expert in Wuhan collected ten thousands animals: capture bats in mountain at night. Changjiang Times 2017. 8. Li QX, Zhanyao. Playing with elephant dung, fishing for sea bottom mud: the work that will change China's future. thepaper 2019. 9. Ge XY, Li JL, Yang XL, et al. Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor. Nature 2013; 503(7477): 535-8. 10. Menachery VD, Yount BL, Jr., Debbink K, et al. A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence. Nature medicine 2015; 21(12): 1508-13.
这算是权威人士发话吧，如果信口雌黄不是要毁了一世英名。
Author and Research Scientist James Lyons-Weiler, PhD – Extended Biography
https://jameslyonsweiler.com/author-and-research-scientist-james-lyons-weiler-phd-extended-biography/
James then found a position at UMASS Lowell, where he founded one of the world’s first Centers for Bioinformatics. He helped develop that institutions’ undergraduate and graduate studies curriculum in Bioinformatics. He routinely had 20 or more students performing analyses for research projects, asking questions about the impact of algorithm selection on the biological inferences being made in many types of diseases. He produced online software that implemented numerous methods of microarray data analysis – and that provided objective performance evaluation measures to aid in the selection of the optimal methods. He studied gene expression in cancer, publishing a paper on overcoming experimental design flaws such as confounded controls. He taught courses in biology, genetics, and bioinformatics, and set up a sequencing lab for the Department of Biological Sciences.
But both Rolling Stone and The Skeptical Raptor view James Lyons-Weiler as an anti-vaxxer, so he might not be too conversant about medical matters either.
Since he is an anti-vaxxer, any medical opinion Lyons-Weiler utters will require quadruple verification from non-vaxxer scientists before I believe it.
It is interesting that his theory about the origins coronavirus portrays vaccine makers as villains. It would make a good plot for an end of the world movie, but as science there is a 99.999 percent chance it is utter crap.
我不具备这方面的专业知识。
https://sciencebasedmedicine.org/james-lyons-weiler-coronavirus-conspiracy-vaccine/
https://healthfeedback.org/claimreview/2019-novel-coronavirus-2019-ncov-does-not-contain-pshuttle-sn-sequence-no-evidence-that-virus-is-man-made/
外果仁也在吵啊
https://londonlovesbusiness.com/chinese-scientists-reveal-true-cause-of-coronavirus-outbreak-in-wuhan/
真正的被蝙蝠袭击应该是被蝙蝠抓、咬皮肤。
https://web.archive.org/web/20200214144447/https://www.researchgate.net/publication/339070128_The_possible_origins_of_2019-nCoV_coronavirus
我觉得文章中用pShuttle-SN complete sequence 去做blast 不对。当pShuttle-SN 和Vira vector recombine 了以后, pShuttle-SN 的大部分sequence 都消失了。这样做出的结果没有用。他们应该用ITR-SN-ITRsequence 去blast。
Download citation Share Download full-text PDF The possible origins of 2019-nCoV coronavirus Preprint (PDF Available) · February 2020 with 547 Reads DOI: 10.13140/RG.2.2.21799.29601 Cite this publication
Content uploaded by Botao Xiao Author content Content may be subject to copyright. Download full-text PDF The possible origins of 2019-nCoV coronavirus Botao Xiao1,2* and Lei Xiao3 1 Joint International Research Laboratory of Synthetic Biology and Medicine, School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China 2 School of Physics, Huazhong University of Science and Technology, Wuhan 430074, China 3 Tian You Hospital, Wuhan University of Science and Technology, Wuhan 430064, China * Corresponding author: [email protected] Tel / Fax: 86-20-3938-0631 The 2019-nCoV coronavirus has caused an epidemic of 28,060 laboratory-confirmed infections in human including 564 deaths in China by February 6, 2020. Two descriptions of the virus published on Nature this week indicated that the genome sequences from patients were 96% or 89% identical to the Bat CoV ZC45 coronavirus originally found in Rhinolophus affinis 1,2. It was critical to study where the pathogen came from and how it passed onto human. An article published on The Lancet reported that 41 people in Wuhan were found to have the acute respiratory syndrome and 27 of them had contact with Huanan Seafood Market 3. The 2019-nCoV was found in 33 out of 585 samples collected in the market after the outbreak. The market was suspicious to be the origin of the epidemic, and was shut down according to the rule of quarantine the source during an epidemic. The bats carrying CoV ZC45 were originally found in Yunnan or Zhejiang province, both of which were more than 900 kilometers away from the seafood market. Bats were normally found to live in caves and trees. But the seafood market is in a densely-populated district of Wuhan, a metropolitan of ~15 million people. The probability was very low for the bats to fly to the market. According to municipal reports and the testimonies of 31 residents and 28 visitors, the bat was never a food source in the city, and no bat was traded in the market. There was possible natural recombination or intermediate host of the coronavirus, yet little proof has been reported. Was there any other possible pathway? We screened the area around the seafood market and identified two laboratories conducting research on bat coronavirus. Within ~280 meters from the market, there was the Wuhan Center for Disease Control & Prevention (WHCDC) (Figure 1, from Baidu and Google maps). WHCDC hosted animals in laboratories for research purpose, one of which was specialized in pathogens collection and identification 4- 6. In one of their studies, 155 bats including Rhinolophus affinis were captured in Hubei province, and other 450 bats were captured in Zhejiang province 4. The expert in collection was noted in the Author Contributions (JHT). Moreover, he was broadcasted for collecting viruses on nation-wide newspapers and websites in 2017 and 2019 7,8. He described that he was once by attacked by bats and the blood of a bat shot on his skin. He knew the extreme danger of the infection so he quarantined himself for 14 days 7. In another accident, he quarantined himself again because bats peed on him. He was once thrilled for capturing a bat carrying a live tick 8. Surgery was performed on the caged animals and the tissue samples were collected for DNA and RNA extraction and sequencing 4, 5. The tissue samples and contaminated trashes were source of pathogens. They were only ~280 meters from the seafood market. The WHCDC was also adjacent to the Union Hospital (Figure 1, bottom) where the first group of doctors were infected during this epidemic. It is plausible that the virus leaked around and some of them contaminated the initial patients in this epidemic, though solid proofs are needed in future study. The second laboratory was ~12 kilometers from the seafood market and belonged to Wuhan Institute of Virology, Chinese Academy of Sciences 1, 9, 10. This laboratory reported that the Chinese horseshoe bats were natural reservoirs for the severe acute respiratory syndrome coronavirus (SARS-CoV) which caused the 2002-3 pandemic 9. The principle investigator participated in a project which generated a chimeric virus using the SARS-CoV reverse genetics system, and reported the potential for human emergence 10. A direct speculation was that SARS-CoV or its derivative might leak from the laboratory. In summary, somebody was entangled with the evolution of 2019-nCoV coronavirus. In addition to origins of natural recombination and intermediate host, the killer coronavirus probably originated from a laboratory in Wuhan. Safety level may need to be reinforced in high risk biohazardous laboratories. Regulations may be taken to relocate these laboratories far away from city center and other densely populated places. Contributors BX designed the comment and performed literature search. All authors performed data acquisition and analysis, collected documents, draw the figure, and wrote the papers. Acknowledgements This work is supported by the National Natural Science Foundation of China (11772133, 11372116). Declaration of interests All authors declare no competing interests. References 1. Zhou P, Yang X-L, Wang X-G, et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature 2020. https://doi.org/10.1038/s41586-020-2012-7. 2. Wu F, Zhao S, Yu B, et al. A new coronavirus associated with human respiratory disease in China. Nature 2020. https://doi.org/10.1038/s41586-020-2008-3. 3. Huang C, Wang Y, Li X, et al. Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. The Lancet 2019. https://doi.org/10.1016/S0140- 6736(20)30183-5. 4. Guo WP, Lin XD, Wang W, et al. Phylogeny and origins of hantaviruses harbored by bats, insectivores, and rodents. PLoS pathogens 2013; 9(2): e1003159. 5. Lu M, Tian JH, Yu B, Guo WP, Holmes EC, Zhang YZ. Extensive diversity of rickettsiales bacteria in ticks from Wuhan, China. Ticks and tick-borne diseases 2017; 8(4): 574-80. 6. Shi M, Lin XD, Chen X, et al. The evolutionary history of vertebrate RNA viruses. Nature 2018; 556(7700): 197-202. 7. Tao P. Expert in Wuhan collected ten thousands animals: capture bats in mountain at night. Changjiang Times 2017. 8. Li QX, Zhanyao. Playing with elephant dung, fishing for sea bottom mud: the work that will change China's future. thepaper 2019. 9. Ge XY, Li JL, Yang XL, et al. Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor. Nature 2013; 503(7477): 535-8. 10. Menachery VD, Yount BL, Jr., Debbink K, et al. A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence. Nature medicine 2015; 21(12): 1508-13.他不明白应该用什么去做blast。是否是人工合成的证据不在68%上。要比的是病毒和pShuttle-SN的ITR 附近的同源性。我想James Lyons Weiler是不会傻到用pShuttle-SN complete vector 去做alignment的，懂得病毒是怎样clone出来的都不会这样去blast。
不专业