一个广为接受的事实：LAG3 （a checkpoint molecule), 不管是否在别的细胞上表达 与否, 应该至少在小胶质细胞，免疫细胞上表达，这个Johns Hopkins, Ted Dawson and Xiaobo Mao 2016年‘有大发现’，发了一篇 Science，“Pathological α- synuclein transmission initiated by binding lymphocyte-activation gene 3” 【Science (2016) pubmed: 27708076，doi: 10.1126/science.aah3374】，声称 ‘ LAG3 was primarily expressed in neurons because LAG3 was not detectable in astrocytes or microglia’. 审稿人水平也太不敢恭维了。
同一个组同一位一作，估计是看到2016年的他们自己的发现难以自圆谎（？），Xiaobo Mao 在一篇新的预印版 they show the expression of LAG3 in microglia. (Aplp1 and the Aplp1-Lag3 Complex facilitates transmission of pathologic α- synuclein, https:// www.biorxiv.org/content/10.1101/2021.05.01.442157v1)，翻手为云覆手为雨啊。 看来发大paper 拿到 grant 是王道，哪管事情真伪啊。
现在是一个瑞士的实验组发现 LAG3 不但不在神经元上表达，而且发现 LAG3根本就没 有这个Johns Hopkins, Ted Dawson and Xiaobo Mao 发现的调节synucleinopathies的作用， LAG3 is not expressed in human and murine neurons and does not modulate α- synucleinopathies" (https://www.biorxiv.org/content/10.1101/2021.04.25. 441302v1 )
The same group and the same first author, they claimed in their 2016 Sciencepaper, that ‘LAG3 was primarily expressed in neurons because LAG3 was not detectable in astrocytes or microglia’. (Science. 2016 Sep 30; 353(6307): aah3374),
So many good labs had CLEARLY shown and approved that LAG3 is thought to be largely in microglia. If the LAG3 antibodies they used were specific, LAG3 expression in microglia should be detected in their 2016 Science paper, why they say "LAG3 was expressed in neurons but not in microglia?
Now, Mao et al NEW preprint, they show the expression of LAG3 in microglia. (Aplp1 and the Aplp1-Lag3 Complex facilitates transmission of pathologic α-synuclein, https://www.biorxiv.org/content/10.1101/2021.05.01.442157v1 )
And interestingly, in this preprint (Aplp1 and the Aplp1-Lag3 Complex facilitates transmission of pathologic α-synuclein, https://www.biorxiv.org/content/10.1101/2021.05.01.442157v1 ), using LAG3-YFP transgenic mice to verify LAG3 protein expression in (mouse) neuron look good, but why the GFP antibody was used to stain YFP? Artifacts? The anti-GFP staining pattern wasnot convincing, if you look their nuclei counterstaining, it seems very like auto-fluorescence. Can this truly approve the neuronal expression of LAG3, why not do solid and convincing studies like another preprint "LAG3 isnot expressed in human and murine neurons and does not modulate α- synucleinopathies" (https://www.biorxiv.org/content/10.1101/2021.04.25. 441302v1 )
"The immunoreactivity of anti-GFP, which recognizes YFP co-localized with MAP2 immunostaining in the spinal cord, olfactory bulb, striatum, substantianigra pars compacta, hippocampus, cortex, cerebellum and brainstem (fig. S3A). The lack of immunoreactivity of anti-GFP in WT mice indicates that theanti-GFP immunoreactivity is specific in Lag3L/L-YFP mice (fig. S3B). In addition, anti-GFP colocalizes with IBA1 immunostaining, but not GFAP immunostaining (fig. S3B)"
【 在 benature (haha) 的大作中提到: 】 : 标 题: 翻手为云覆手为雨, Johns Hopkins, Ted Dawson et al. : 发信站: BBS 未名空间站 (Sun May 16 14:48:29 2021, 美东) : : 生物学界的高水平造假，看大家如何编故事，讲故事好听的吗？ : : 一个广为接受的事实：LAG3 （a checkpoint molecule), 不管是否在别的细胞上表达 : 与否, 应该至少在小胶质细胞，免疫细胞上表达，这个Johns Hopkins, Ted Dawson : and Xiaobo Mao 2016年‘有大发现’，发了一篇 Science，“Pathological α- : synuclein transmission initiated by binding lymphocyte-activation gene 3” : 【Science (2016) pubmed: 27708076，doi: 10.1126/science.aah3374】，声称 ‘: LAG3 was primarily expressed in neurons because LAG3 was not detectable in : astrocytes or microglia’. 审稿人水平也太不敢恭维了。 : : 同一个组同一位一作，估计是看到2016年的他们自己的发现难以自圆谎（？）， Xiaobo : Mao 在一篇新的预印版 they show the expression of LAG3 in microglia. ( Aplp1 : and the Aplp1-Lag3 Complex facilitates transmission of pathologic α- : synuclein, https:// : www.biorxiv.org/content/10.1101/2021.05.01.442157v1)，翻手为云覆手为雨啊。: 看来发大paper 拿到 grant 是王道，哪管事情真伪啊。 : : 现在是一个瑞士的实验组发现 LAG3 不但不在神经元上表达，而且发现 LAG3根本就没 : 有这个Johns Hopkins, Ted Dawson and Xiaobo Mao 发现的调节synucleinopathies的 : 作用， LAG3 is : not expressed in human and murine neurons and does not modulate α- : synucleinopathies" (https://www.biorxiv.org/content/10.1101/2021.04.25. : 441302v1 ) : : ----------------------------------------------------------------------------- : https://pubpeer.com/publications/6DE486C75FB8FC5C057D72B27C926E : : The same group and the same first author, they claimed in their 2016 Science : paper, that ‘LAG3 was primarily expressed in neurons because LAG3 was not: detectable in astrocytes or microglia’. (Science. 2016 Sep 30; 353(6307):: aah3374), : : So many good labs had CLEARLY shown and approved that LAG3 is thought to be : largely in microglia. If the LAG3 antibodies they used were specific, LAG3: expression in microglia should be detected in their 2016 Science paper, why : they say "LAG3 was expressed in neurons but not in microglia? : : Now, Mao et al NEW preprint, they show the expression of LAG3 in microglia. : (Aplp1 and the Aplp1-Lag3 Complex facilitates transmission of pathologic α- : synuclein, https://www.biorxiv.org/content/10.1101/2021.05.01.442157v1 ) : : And interestingly, in this preprint (Aplp1 and the Aplp1-Lag3 Complex : facilitates transmission of pathologic α-synuclein, https://www.biorxiv. org : /content/10.1101/2021.05.01.442157v1 ), using LAG3-YFP transgenic mice to : verify LAG3 protein expression in (mouse) neuron look good, but why the GFP : antibody was used to stain YFP? Artifacts? The anti-GFP staining pattern was : not convincing, if you look their nuclei counterstaining, it seems very : like auto-fluorescence. Can this truly approve the neuronal expression of : LAG3, why not do solid and convincing studies like another preprint "LAG3 is : not expressed in human and murine neurons and does not modulate α- : synucleinopathies" (https://www.biorxiv.org/content/10.1101/2021.04.25. : 441302v1 ) : : "The immunoreactivity of anti-GFP, which recognizes YFP co-localized with : MAP2 immunostaining in the spinal cord, olfactory bulb, striatum, substantia : nigra pars compacta, hippocampus, cortex, cerebellum and brainstem (fig. : S3A). The lack of immunoreactivity of anti-GFP in WT mice indicates that the : anti-GFP immunoreactivity is specific in Lag3L/L-YFP mice (fig. S3B). In : addition, anti-GFP colocalizes with IBA1 immunostaining, but not GFAP : immunostaining (fig. S3B)" : : --
【 在 benature (haha) 的大作中提到: 】 : 生物学界的高水平造假，看大家如何编故事，讲故事好听的吗？ : 一个广为接受的事实：LAG3 （a checkpoint molecule), 不管是否在别的细胞上表达 : 与否, 应该至少在小胶质细胞，免疫细胞上表达，这个Johns Hopkins, Ted Dawson : and Xiaobo Mao 2016年‘有大发现’，发了一篇 Science，“Pathological α- : synuclein transmission initiated by binding lymphocyte-activation gene 3” : LAG3 was primarily expressed in neurons because LAG3 was not detectable in : astrocytes or microglia’. 审稿人水平也太不敢恭维了。 : 同一个组同一位一作，估计是看到2016年的他们自己的发现难以自圆谎（？）， Xiaobo : Mao 在一篇新的预印版 they show the expression of LAG3 in microglia. ( Aplp1 : and the Aplp1-Lag3 Complex facilitates transmission of pathologic α- : ...................
生物学界的高水平造假，看大家如何编故事，讲故事好听的吗？
一个广为接受的事实：LAG3 （a checkpoint molecule), 不管是否在别的细胞上表达
与否, 应该至少在小胶质细胞，免疫细胞上表达，这个Johns Hopkins, Ted Dawson
and Xiaobo Mao 2016年‘有大发现’，发了一篇 Science，“Pathological α-
synuclein transmission initiated by binding lymphocyte-activation gene 3”
【Science (2016) pubmed: 27708076，doi: 10.1126/science.aah3374】，声称 ‘
LAG3 was primarily expressed in neurons because LAG3 was not detectable in
astrocytes or microglia’. 审稿人水平也太不敢恭维了。
同一个组同一位一作，估计是看到2016年的他们自己的发现难以自圆谎（？），Xiaobo Mao 在一篇新的预印版 they show the expression of LAG3 in microglia. (Aplp1 and the Aplp1-Lag3 Complex facilitates transmission of pathologic α-
synuclein, https://
www.biorxiv.org/content/10.1101/2021.05.01.442157v1)，翻手为云覆手为雨啊。
看来发大paper 拿到 grant 是王道，哪管事情真伪啊。
现在是一个瑞士的实验组发现 LAG3 不但不在神经元上表达，而且发现 LAG3根本就没
有这个Johns Hopkins, Ted Dawson and Xiaobo Mao 发现的调节synucleinopathies的作用， LAG3 is
not expressed in human and murine neurons and does not modulate α-
synucleinopathies" (https://www.biorxiv.org/content/10.1101/2021.04.25.
441302v1 )
-----------------------------------------------------------------------------https://pubpeer.com/publications/6DE486C75FB8FC5C057D72B27C926E
The same group and the same first author, they claimed in their 2016 Sciencepaper, that ‘LAG3 was primarily expressed in neurons because LAG3 was not
detectable in astrocytes or microglia’. (Science. 2016 Sep 30; 353(6307):
aah3374),
So many good labs had CLEARLY shown and approved that LAG3 is thought to be
largely in microglia. If the LAG3 antibodies they used were specific, LAG3
expression in microglia should be detected in their 2016 Science paper, why
they say "LAG3 was expressed in neurons but not in microglia?
Now, Mao et al NEW preprint, they show the expression of LAG3 in microglia.
(Aplp1 and the Aplp1-Lag3 Complex facilitates transmission of pathologic α-synuclein, https://www.biorxiv.org/content/10.1101/2021.05.01.442157v1 )
And interestingly, in this preprint (Aplp1 and the Aplp1-Lag3 Complex
facilitates transmission of pathologic α-synuclein, https://www.biorxiv.org/content/10.1101/2021.05.01.442157v1 ), using LAG3-YFP transgenic mice to
verify LAG3 protein expression in (mouse) neuron look good, but why the GFP
antibody was used to stain YFP? Artifacts? The anti-GFP staining pattern wasnot convincing, if you look their nuclei counterstaining, it seems very
like auto-fluorescence. Can this truly approve the neuronal expression of
LAG3, why not do solid and convincing studies like another preprint "LAG3 isnot expressed in human and murine neurons and does not modulate α-
synucleinopathies" (https://www.biorxiv.org/content/10.1101/2021.04.25.
441302v1 )
"The immunoreactivity of anti-GFP, which recognizes YFP co-localized with
MAP2 immunostaining in the spinal cord, olfactory bulb, striatum, substantianigra pars compacta, hippocampus, cortex, cerebellum and brainstem (fig.
S3A). The lack of immunoreactivity of anti-GFP in WT mice indicates that theanti-GFP immunoreactivity is specific in Lag3L/L-YFP mice (fig. S3B). In
addition, anti-GFP colocalizes with IBA1 immunostaining, but not GFAP
immunostaining (fig. S3B)"
编造这个意义何在？
生物千老随便泡个脚就能证明蛋白有没有吧
作假做的水平高点不好吗？
盹盹盹
【 在 benature (haha) 的大作中提到: 】
: 标 题: 翻手为云覆手为雨, Johns Hopkins, Ted Dawson et al.
: 发信站: BBS 未名空间站 (Sun May 16 14:48:29 2021, 美东)
:
: 生物学界的高水平造假，看大家如何编故事，讲故事好听的吗？
:
: 一个广为接受的事实：LAG3 （a checkpoint molecule), 不管是否在别的细胞上表达
: 与否, 应该至少在小胶质细胞，免疫细胞上表达，这个Johns Hopkins, Ted Dawson : and Xiaobo Mao 2016年‘有大发现’，发了一篇 Science，“Pathological α-
: synuclein transmission initiated by binding lymphocyte-activation gene 3”
: 【Science (2016) pubmed: 27708076，doi: 10.1126/science.aah3374】，声称 ‘: LAG3 was primarily expressed in neurons because LAG3 was not detectable in
: astrocytes or microglia’. 审稿人水平也太不敢恭维了。
:
: 同一个组同一位一作，估计是看到2016年的他们自己的发现难以自圆谎（？），
Xiaobo
: Mao 在一篇新的预印版 they show the expression of LAG3 in microglia. (
Aplp1
: and the Aplp1-Lag3 Complex facilitates transmission of pathologic α-
: synuclein, https://
: www.biorxiv.org/content/10.1101/2021.05.01.442157v1)，翻手为云覆手为雨啊。: 看来发大paper 拿到 grant 是王道，哪管事情真伪啊。
:
: 现在是一个瑞士的实验组发现 LAG3 不但不在神经元上表达，而且发现 LAG3根本就没
: 有这个Johns Hopkins, Ted Dawson and Xiaobo Mao 发现的调节synucleinopathies的
: 作用， LAG3 is
: not expressed in human and murine neurons and does not modulate α-
: synucleinopathies" (https://www.biorxiv.org/content/10.1101/2021.04.25.
: 441302v1 )
:
: -----------------------------------------------------------------------------
: https://pubpeer.com/publications/6DE486C75FB8FC5C057D72B27C926E
:
: The same group and the same first author, they claimed in their 2016
Science
: paper, that ‘LAG3 was primarily expressed in neurons because LAG3 was not: detectable in astrocytes or microglia’. (Science. 2016 Sep 30; 353(6307):: aah3374),
:
: So many good labs had CLEARLY shown and approved that LAG3 is thought to
be
: largely in microglia. If the LAG3 antibodies they used were specific, LAG3: expression in microglia should be detected in their 2016 Science paper,
why
: they say "LAG3 was expressed in neurons but not in microglia?
:
: Now, Mao et al NEW preprint, they show the expression of LAG3 in microglia.
: (Aplp1 and the Aplp1-Lag3 Complex facilitates transmission of pathologic
α-
: synuclein, https://www.biorxiv.org/content/10.1101/2021.05.01.442157v1 )
:
: And interestingly, in this preprint (Aplp1 and the Aplp1-Lag3 Complex
: facilitates transmission of pathologic α-synuclein, https://www.biorxiv.
org
: /content/10.1101/2021.05.01.442157v1 ), using LAG3-YFP transgenic mice to
: verify LAG3 protein expression in (mouse) neuron look good, but why the
GFP
: antibody was used to stain YFP? Artifacts? The anti-GFP staining pattern
was
: not convincing, if you look their nuclei counterstaining, it seems very
: like auto-fluorescence. Can this truly approve the neuronal expression of
: LAG3, why not do solid and convincing studies like another preprint "LAG3 is
: not expressed in human and murine neurons and does not modulate α-
: synucleinopathies" (https://www.biorxiv.org/content/10.1101/2021.04.25.
: 441302v1 )
:
: "The immunoreactivity of anti-GFP, which recognizes YFP co-localized with
: MAP2 immunostaining in the spinal cord, olfactory bulb, striatum,
substantia
: nigra pars compacta, hippocampus, cortex, cerebellum and brainstem (fig.
: S3A). The lack of immunoreactivity of anti-GFP in WT mice indicates that
the
: anti-GFP immunoreactivity is specific in Lag3L/L-YFP mice (fig. S3B). In
: addition, anti-GFP colocalizes with IBA1 immunostaining, but not GFAP
: immunostaining (fig. S3B)"
:
: --
为了名和利吧： fame and money，学术界不就是教授的title，grant。
虽然这个发现赶不上韩春雨的NgAgo, 但是也是大发现啊，对于一个以 synuclein 为病理特征的帕金森氏疾病是一个巨大突破，想想只要阻断这个LAG3，不就搞定了吗？
【 在 rihai (海桑虎桑柱桑等倭杂之克星) 的大作中提到: 】
: 编造这个意义何在？
: 生物千老随便泡个脚就能证明蛋白有没有吧
: 作假做的水平高点不好吗？
: 盹盹盹
: Xiaobo
: Aplp1
: ---
: Science
: be
: why
: ...................
没有被造假举报吗
这种在学术圈不是很常见吗？PI或者第一作者因为某次偶然的机会发现了一个有意思的现象，接下来设计一堆实验来验证。如果现象是真的那绝对可以发不错的文章开辟一个新的研究方向。但大部分时候这个现象只是假的巧合，如果PI或者第一作者认死理，所有实验都将基于这个假结果，每次都挑符合假设想的结果。大牛实验室发大文章，小牛屁都发不了。
【 在 benature (haha) 的大作中提到: 】
: 生物学界的高水平造假，看大家如何编故事，讲故事好听的吗？
: 一个广为接受的事实：LAG3 （a checkpoint molecule), 不管是否在别的细胞上表达
: 与否, 应该至少在小胶质细胞，免疫细胞上表达，这个Johns Hopkins, Ted Dawson : and Xiaobo Mao 2016年‘有大发现’，发了一篇 Science，“Pathological α-
: synuclein transmission initiated by binding lymphocyte-activation gene 3”
: LAG3 was primarily expressed in neurons because LAG3 was not detectable in
: astrocytes or microglia’. 审稿人水平也太不敢恭维了。
: 同一个组同一位一作，估计是看到2016年的他们自己的发现难以自圆谎（？），
Xiaobo
: Mao 在一篇新的预印版 they show the expression of LAG3 in microglia. (
Aplp1
: and the Aplp1-Lag3 Complex facilitates transmission of pathologic α-
: ...................
这个算是编造故事吧
【 在 ElonMusk ($TSLA (准备亏废）) 的大作中提到: 】
: 没有被造假举报吗