目前全美最常用的临床呼吸类病毒PCR诊断所用仪器可以检测四到五种冠状病毒, 包括HCoV-229E, -NL63, –OC43, -HKU1, and MERS-CoV。但是,新冠和这些冠状病毒的相似度不高,PCR 所用引物无法检测到新冠。目前很多医院的送检标准是排除流感和其他病毒的情况下根据临床表现送检样本到CDC.
Link:https://www.sciencedirect.com/science/article/pii/S1567134820300435 “ To date, 2019-nCoV has been detected in human clinical specimens by next-generation sequencing, real-time RT-PCR, cell culture, and electron microscopy (Zhu et al., 2020). CDC recommends that clinical virology laboratories should not attempt viral isolation from specimens collected from 2019-nCoV patients under investigation (PUI). At this time, diagnostic testing for 2019-nCoV can be conducted only at CDC (https://www.cdc.gov/coronavirus/2019-nCoV/guidelines-clinical-specimens.html). Because 2019-nCoV is a newly discovered virus, the spectrum of the available diagnostic tools is tight. At present, there are several commercially available multiplex NAAT tests for the detection of pathogenic organisms in respiratory specimens in cliniHCoV-229E, -NL63, –OC43, -HKU1, and MERS-CoVcal virology laboratories (Beckmann and Hirsch, 2016; Huang et al., 2018, Babady et al., 2018). They can detect HCoV-229E, -NL63, –OC43, and -HKU1. In addition, the BioFire FilmArray Respiratory Panel 2 plus and the BioFire FilmArray Pneumonia Panel plus can detect MERS-CoV in human clinical specimens. A recent study reported that the RespiFinderSmart22kit (PathoFinder BV, Netherlands) failed to identify 2019-nCoV in the bronchoalveolar-lavage specimens collected from 2019-nCoV infected patients in Wuhan, China (Zhu et al., 2020). Two major FDA-cleared multiplex PCR systems in the United States, including the ePlex Respiratory Pathogen Panel (GenMark Diagnostics, Carlsbad, CA) and the FilmArray Coronavirus Assays (BioFire Diagnostics, Salt Lake City, UT), are predicted no cross-reactivity with 2019-nCoV. Our pair-wise sequence analysis demonstrated that 2019-nCoV shared very low nucleotide similarity, less than 50%, to HCoV-229E, -NL63, –OC43, -HKU1, and MERS-CoV。”
Link:https://www.sciencedirect.com/science/article/pii/S1567134820300435
“ To date, 2019-nCoV has been detected in human clinical specimens by next-generation sequencing, real-time RT-PCR, cell culture, and electron microscopy (Zhu et al., 2020). CDC recommends that clinical virology laboratories should not attempt viral isolation from specimens collected from 2019-nCoV patients under investigation (PUI). At this time, diagnostic testing for 2019-nCoV can be conducted only at CDC (https://www.cdc.gov/coronavirus/2019-nCoV/guidelines-clinical-specimens.html). Because 2019-nCoV is a newly discovered virus, the spectrum of the available diagnostic tools is tight. At present, there are several commercially available multiplex NAAT tests for the detection of pathogenic organisms in respiratory specimens in cliniHCoV-229E, -NL63, –OC43, -HKU1, and MERS-CoVcal virology laboratories (Beckmann and Hirsch, 2016; Huang et al., 2018, Babady et al., 2018). They can detect HCoV-229E, -NL63, –OC43, and -HKU1. In addition, the BioFire FilmArray Respiratory Panel 2 plus and the BioFire FilmArray Pneumonia Panel plus can detect MERS-CoV in human clinical specimens. A recent study reported that the RespiFinderSmart22kit (PathoFinder BV, Netherlands) failed to identify 2019-nCoV in the bronchoalveolar-lavage specimens collected from 2019-nCoV infected patients in Wuhan, China (Zhu et al., 2020). Two major FDA-cleared multiplex PCR systems in the United States, including the ePlex Respiratory Pathogen Panel (GenMark Diagnostics, Carlsbad, CA) and the FilmArray Coronavirus Assays (BioFire Diagnostics, Salt Lake City, UT), are predicted no cross-reactivity with 2019-nCoV. Our pair-wise sequence analysis demonstrated that 2019-nCoV shared very low nucleotide similarity, less than 50%, to HCoV-229E, -NL63, –OC43, -HKU1, and MERS-CoV。”
开始时说白人黑人不易感,根据伊朗(也有那么点可能是MSERS)和意大利的情况来看,可能不是这样
真的没有必要检测。不要神经兮兮。
美国是专家治国。
我说过要cdc改protocol除非是像韩国那样感染一大片。不付出血的代价都不行。
意大利在呼吸科这方面一直挺领先的其实。。。美帝药领先 肿瘤什么的也很厉害 但是普通医生接触乱七八糟的病的频率真心比较低。。。
单纯说一下肺炎吃一种抗生素不起效的情况,很可能是antibiotic resistance, 我家小孩经历过,换成第四种抗生素才真正起效,隔壁的贴子是哪个,我想去看看
网友急疯了,可以理解,因为怕是新冠,怕传到自个儿这儿来么。
孩子妈妈急疯了,那是被网上弄傻了吧?肺炎严重了,重点难点不是怎么治,而是要确认自个儿孩子是不是属于那个数据点?如果确认了是新冠,就有准确的治疗方案,倒可以理解,事实是这样么?不还是没有特别手段,照病毒性肺炎一样处理么。
至少可以住院隔离吧?还可以吃给西雅图病人吃的那个药。
隔离问题:就是说孩子妈妈急疯了,是因为怕孩子传染给别人?这儿网友如果这么想我确实可以理解。。。
至于“西雅图病人吃的那个药”,我确实不清楚:
1. 这个药是否确认(或者很可能)对新冠有效。
2. 对于“非新冠”的肺炎,如果严重到西雅图病人那个程度,医院会不会也给这个药。
这两点不清楚的话,确认是不是新冠,对孩子有任何帮助么?
也不一定是怕传染给别人。毕竟能住院(而且因为新冠保险肯定给报,没准还免费),就比在家呆着安全,对病人而言。
对家属而言更是了,没准孩子还有兄弟姐妹,那妈妈也怕传染给自家人。