The oligonucleotide primers and probes for detection of 2019-nCoV were selected from regions of the virus nucleocapsid (N) gene. The panel is designed for specific detection of SARS-CoV-2 (two primer/probe sets). An additional primer/probe set to detect the human RNase P gene (RP) in control samples and clinical specimens is also included in the panel.
spike protein mRNA sequence, I don't mean to say the spike protein peptide sequence. you can never detect any protein by RT-PCR test. you might detect S protein by ELISA assay.
https://zh.wikipedia.org/wiki/%E8%81%9A%E5%90%88%E9%85%B6%E9%93%BE%E5%BC%8F%E5%8F%8D%E5%BA%94
变性达到消毒作用.引物不是引S蛋白.
如果我解释多聚酶链反应原理和引物选择的原理，肯定会被骂“理论砖家”。但是您的问题确实说的非常不明白。
疫苗公司应该补测一批人打完疫苗后检测阳性是啥原因?
rt-pcr, ELISA assay 都是最常见的生物学测试。covid test测的是核酸，不是蛋白，和spike 大小没有关系。
在多说一句吧，现在测covid19核酸检测测的一种核鞘蛋白virus nucleocapsid (N)。既不测spike 蛋白也不测S protein的 mRNA
The oligonucleotide primers and probes for detection of 2019-nCoV were selected from regions of the virus nucleocapsid (N) gene. The panel is designed for specific detection of SARS-CoV-2 (two primer/probe sets). An additional primer/probe set to detect the human RNase P gene (RP) in control samples and clinical specimens is also included in the panel.
spike protein mRNA sequence, I don't mean to say the spike protein peptide sequence. you can never detect any protein by RT-PCR test. you might detect S protein by ELISA assay.
测核酸需要用引物扩增，测蛋白质一般用有关的抗体，没有引物可以扩增。
另外，新冠病毒再小，也比S蛋白大，因为S蛋白只是新冠病毒的一部分，很小的一部分。