Thus, functionally characterized members of families 1, 5, and 7 are almost without exception specific for sugars; characterized members of families 2 and 3 are without exception specific for drugs and other deleterious substances; and families 4, 6, 8, 9, 11 to 14, and 17 are specific for various classes of anionic compounds. Furthermore, the only nucleoside permeases in the MFS are found in family 10, and most of the aromatic acid permeases are found in family 15. These observations clearly show that substrate specificity is a well-conserved trait and that phylogenetic classification provides a limited but reliable guide to function.
Similar considerations can be applied to pump polarity. Thus, while members of families 1, 5, 7, and 8 can apparently function quite readily by one or more modes (e.g., uniport, symport with inwardly directed polarity, and/or antiport), families 2, 3, 4, and 11 apparently function with a high propensity for an antiport mechanism, and families 6, 9, 10, and 12 to 15 probably function with a high propensity for a cation symport mechanism. Clearly, these mechanistic differences must reflect structural and catalytic residue differences, regardless of whether they reflect qualitative or quantitative differences. The molecular bases for these differences should be subject to biochemical, biophysical, and molecular genetic analyses.
她在国内的电视节目里公开讲她的团队破解了葡萄糖载体的机理。这是明摆着欺世盗名。与是否引用文章还不是一个层次的。她如果告诉审稿人，葡萄糖载体的机理早就被破解了，那她的论文是不可能被顶级杂志接收的，她也无法在电视上说是她破解了这个机理。通过她利用审稿人与投稿人的互信机制，她达到的目的是彻头彻尾的欺世盗名---葡萄糖载体的机理是她破解的。事实上，她只是证明了我的结论是正确的。
跟阎的UhpT差距比较大．
假如你那算破解结构，你比颜早破解20年，为啥没多少人留意你的工作？ 你那只多算一个结构猜想吧，我也没细看，但知道你用突变方法，我猜测你拿不到确实的结构。
参合
扔扔瓜皮
我扔哪儿了，我找个垃圾桶行不
除非润涛的机理只是整个机理很小的部分。
具体见:
• 颜宁2012年nature一文对前人文献的引用. - SwiperTheFox -
(1837 bytes) (117 reads) 05/15/2019
Thus, functionally characterized members of families 1, 5, and 7 are almost without exception specific for sugars; characterized members of families 2 and 3 are without exception specific for drugs and other deleterious substances; and families 4, 6, 8, 9, 11 to 14, and 17 are specific for various classes of anionic compounds. Furthermore, the only nucleoside permeases in the MFS are found in family 10, and most of the aromatic acid permeases are found in family 15. These observations clearly show that substrate specificity is a well-conserved trait and that phylogenetic classification provides a limited but reliable guide to function.
Similar considerations can be applied to pump polarity. Thus, while members of families 1, 5, 7, and 8 can apparently function quite readily by one or more modes (e.g., uniport, symport with inwardly directed polarity, and/or antiport), families 2, 3, 4, and 11 apparently function with a high propensity for an antiport mechanism, and families 6, 9, 10, and 12 to 15 probably function with a high propensity for a cation symport mechanism. Clearly, these mechanistic differences must reflect structural and catalytic residue differences, regardless of whether they reflect qualitative or quantitative differences. The molecular bases for these differences should be subject to biochemical, biophysical, and molecular genetic analyses.
你从功能去推结构，比较困难，大家脑袋中没有那个三维构象。所以只能算瞎子摸象。
阎的一家之言?
老阎呀，你知道你的影响力。
三维空间构象就是那个Picture.活体构象拿不到罢了，搞一个死图像。但总比没有图像好。