The Rebel Patient™: Study Shows Chicken Egg Yolk Antibodies Stop Spike Protein from Attaching to AcE2 Receptors *****RN82L48vfs
— ? Dr Margaret Aranda Ferrante, MD PhD FACFEI ? (@TheRebelPatient) February 2, 2023
National Library of Medicine now says Chicken Egg Yolk Antibodies (IgYs) block the binding of multiple SARS-CoV-2 spike protein variants to human ACE2 pic.twitter.com/QaobL19nfe
The SARS-CoV-2 virus is still spreading worldwide, and there is an urgent need to effectively prevent and control this pandemic. This study evaluated the potential efficacy of Egg Yolk Antibodies (IgY) as a neutralizing agent against the SARS-CoV-2. We investigated the neutralizing effect of anti-spike-S1 IgYs on the SARS-CoV-2 pseudovirus, as well as its inhibitory effect on the binding of the coronavirus spike protein mutants to human ACE2. Our results show that the anti-Spike-S1 IgYs showed significant neutralizing potency against SARS-CoV-2 pseudovirus, various spike protein mutants, and even SARS-CoV in vitro. It might be a feasible tool for the prevention and control of ongoing COVID-19.
“The purified recombinant SARS-CoV-2 S1 protein was mixed and emulsified with Freund's immune adjuvant in equal volume and then used as an immunogen. Each hen was injected (intramuscular) with 150 μg of the recombinant spike protein under the wings, once a week for 4 weeks, and then IgY was extracted and the titer evaluated. ”
AbstractThe SARS-CoV-2 virus is still spreading worldwide, and there is an urgent need to effectively prevent and control this pandemic. This study evaluated the potential efficacy of Egg Yolk Antibodies (IgY) as a neutralizing agent against the SARS-CoV-2. We investigated the neutralizing effect of anti-spike-S1 IgYs on the SARS-CoV-2 pseudovirus, as well as its inhibitory effect on the binding of the coronavirus spike protein mutants to human ACE2. Our results show that the anti-Spike-S1 IgYs showed significant neutralizing potency against SARS-CoV-2 pseudovirus, various spike protein mutants, and even SARS-CoV in vitro. It might be a feasible tool for the prevention and control of ongoing COVID-19.
Keywords: Chicken Egg Yolk Antibodies; IgY; Neutralizing agent; SARS-CoV-2; Spike protein variants.
https://pubmed.ncbi.nlm.nih.gov/33191178/
https://www.sciencedirect.com/science/article/pii/S1567576920336390
蛋黄里的抗体对很多感染有效的研究文章（可以查到很多）：
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106195/
https://www.semanticscholar.org/paper/Protective-Effects-of-Chicken-Egg-Yolk-(IgYs)-Cai-Liu/140876f05a6d9c5f3f43857e85b4fa34bd2b766b
https://www.semanticscholar.org/paper/Chicken-egg-yolk-antibody-(IgY)-as-diagnostics-and-Diraviyam-Ambi/9d655ef345b5a3ce7e422dec30e2e195ff8560e7
或者，看谁家有散养的鸡，买那的鸡蛋。我就是到有机store买鸡蛋。
https://zhuanlan.zhihu.com/p/440363866#:~:text=IgG%E5%92%8CIgY%E4%B8%8D%E5%90%8C%E4%B9%8B%E5%A4%84%EF%BC%9A&text=IgY%E9%93%B0%E9%93%BE%E5%8C%BA%E7%9F%AD%E4%B8%94,%E5%9C%A8%E5%93%BA%E4%B9%B3%E5%8A%A8%E7%89%A9%E4%B8%AD%E5%AE%8C%E6%88%90%E3%80%82
“The purified recombinant SARS-CoV-2 S1 protein was mixed and emulsified with Freund's immune adjuvant in equal volume and then used as an immunogen. Each hen was injected (intramuscular) with 150 μg of the recombinant spike protein under the wings, once a week for 4 weeks, and then IgY was extracted and the titer evaluated. ”
里面的蛋白会被酶消化成氨基酸/肽,然后重新组成人体需要的蛋白,Igy是大分子会被酶消化.
做抗体Igy需要提取纯化...后面好多步骤.
http://www.alphamab.com/content/details_37_461.html