Liu GH#, Qu J, Suzuki K, Nivet E, Li M, Montserrat N, Yi F, Xu X, Ruiz S, Zhang W, Ren B, Wagner U, Kim A, Li Y, Goebl A, Kim J, Soligalla R, Dubova I, Thompson J, Yates J III, Esteban C, Sancho-Martinez I, Belmonte JC#. Progressive degeneration of human neural stem cells caused by pathogenic LRRK2. Nature. 2012; 491(7425):603-7. (#Corresponding author)
(1) Chen X, Xie C, Tian W, et al. Parkinson's disease-related Leucine-rich repeat kinase 2 modulates nuclear morphology and genomic stability in striatal projection neurons during aging. Mol Neurodegener. 2020;15(1):12. Published 2020 Feb 19. doi:10.1186/s13024-020-00360-0
(2) Shani V, Safory H, Szargel R, et al. Physiological and pathological roles of LRRK2 in the nuclear envelope integrity. Hum Mol Genet. 2019; 28( 23):3982-3996. doi:10.1093/hmg/ddz245
(3) Candelario KM, Balaj L, Zheng T, et al. Exosome/microvesicle content is altered in leucine-rich repeat kinase 2 mutant induced pluripotent stem cell-derived neural cells. J Comp Neurol. 2020;528(7):1203-1215. doi:10.1002/cne.24819
(4) Walter J, Bolognin S, Antony PMA, et al. Neural Stem Cells of Parkinson's Disease Patients Exhibit Aberrant Mitochondrial Morphology and Functionality. Stem Cell Reports. 2019;12(5):878-889. doi:10.1016/j.stemcr. 2019.03.004
(5) Lee M, Sim H, Ahn H, et al. Direct Reprogramming to Human Induced Neuronal Progenitors from Fibroblasts of Familial and Sporadic Parkinson's Disease Patients. Int J Stem Cells. 2019;12(3):474-483. doi:10.15283/ ijsc19075
(6) Yoon JH, Mo JS, Kim MY, et al. LRRK2 functions as a scaffolding kinase of ASK1-mediated neuronal cell death. Biochim Biophys Acta Mol Cell Res. 2017;1864(12):2356-2368. doi:10.1016/j.bbamcr.2017.09.001
(7) Le Grand JN, Gonzalez-Cano L, Pavlou MA, Schwamborn JC. Neural stem cells in Parkinson's disease: a role for neurogenesis defects in onset and progression. Cell Mol Life Sci. 2015;72(4):773-797. doi:10.1007/s00018-014- 1774-1
(8) Tsika E, Kannan M, Foo CS, et al. Conditional expression of Parkinson's disease-related R1441C LRRK2 in midbrain dopaminergic neurons of mice causes nuclear abnormalities without neurodegeneration. Neurobiol Dis. 2014;71:345-358. doi:10.1016/j.nbd.2014.08.027
(9) Bahnassawy L, Nicklas S, Palm T, et al. The parkinson's disease- associated LRRK2 mutation R1441G inhibits neuronal differentiation of neural stem cells. Stem Cells Dev. 2013;22(18):2487-2496. doi:10.1089/scd.2013. 0163
(10) Winner B, Melrose HL, Zhao C, et al. Adult neurogenesis and neurite outgrowth are impaired in LRRK2 G2019S mice. Neurobiol Dis. 2011;41(3):706- 716. doi:10.1016/j.nbd.2010.12.008
北京动物所的刘光慧在2012年以通讯作者发表了一篇没有人能够重复出来的Nature文章. 大家可以看看. LRRK2的突变虽然跟帕金森病有关系, 但是其他几乎所有的文章都无
法观察到带有LRRK2点突变的神经干细胞有任何的异常. 很感兴趣刘光慧是怎么做出来
的这篇文章?
我们做帕金森病的都知道这个LRRK2-G2019S并不会对人神经干细胞有任何的影响!
Liu GH#, Qu J, Suzuki K, Nivet E, Li M, Montserrat N, Yi F, Xu X, Ruiz S,
Zhang W, Ren B, Wagner U, Kim A, Li Y, Goebl A, Kim J, Soligalla R, Dubova I, Thompson J, Yates J III, Esteban C, Sancho-Martinez I, Belmonte JC#.
Progressive degeneration of human neural stem cells caused by pathogenic
LRRK2. Nature. 2012; 491(7425):603-7. (#Corresponding author)
虽然刘光慧跟杨辉都是一个德行, 但是造假剽窃这方面比杨辉高明
我对神经领域的新发现特别是近年的新靶点一直很有兴趣，随手PUBMED了一下，发现了一些PD相关LRRK2 mutation（包括G2019S, R1441G等）对人和小鼠的Neural stem/
progenitor cell和neuronal cell的影响的paper，对细胞核膜结构及细胞死亡/凋亡的影响均有报道。不知楼主用的是什么样的研究体系和模式生物？是否愿意share一下您
的方法和data？谢谢
(1) Chen X, Xie C, Tian W, et al. Parkinson's disease-related Leucine-rich
repeat kinase 2 modulates nuclear morphology and genomic stability in
striatal projection neurons during aging. Mol Neurodegener. 2020;15(1):12.
Published 2020 Feb 19. doi:10.1186/s13024-020-00360-0
(2) Shani V, Safory H, Szargel R, et al. Physiological and pathological
roles of LRRK2 in the nuclear envelope integrity. Hum Mol Genet. 2019; 28(
23):3982-3996. doi:10.1093/hmg/ddz245
(3) Candelario KM, Balaj L, Zheng T, et al. Exosome/microvesicle content is altered in leucine-rich repeat kinase 2 mutant induced pluripotent stem cell-derived neural cells. J Comp Neurol. 2020;528(7):1203-1215. doi:10.1002/cne.24819
(4) Walter J, Bolognin S, Antony PMA, et al. Neural Stem Cells of Parkinson's Disease Patients Exhibit Aberrant Mitochondrial Morphology and
Functionality. Stem Cell Reports. 2019;12(5):878-889. doi:10.1016/j.stemcr.
2019.03.004
(5) Lee M, Sim H, Ahn H, et al. Direct Reprogramming to Human Induced
Neuronal Progenitors from Fibroblasts of Familial and Sporadic Parkinson's
Disease Patients. Int J Stem Cells. 2019;12(3):474-483. doi:10.15283/
ijsc19075
(6) Yoon JH, Mo JS, Kim MY, et al. LRRK2 functions as a scaffolding kinase
of ASK1-mediated neuronal cell death. Biochim Biophys Acta Mol Cell Res.
2017;1864(12):2356-2368. doi:10.1016/j.bbamcr.2017.09.001
(7) Le Grand JN, Gonzalez-Cano L, Pavlou MA, Schwamborn JC. Neural stem
cells in Parkinson's disease: a role for neurogenesis defects in onset and
progression. Cell Mol Life Sci. 2015;72(4):773-797. doi:10.1007/s00018-014-
1774-1
(8) Tsika E, Kannan M, Foo CS, et al. Conditional expression of Parkinson's disease-related R1441C LRRK2 in midbrain dopaminergic neurons of mice causes nuclear abnormalities without neurodegeneration. Neurobiol Dis. 2014;71:345-358. doi:10.1016/j.nbd.2014.08.027
(9) Bahnassawy L, Nicklas S, Palm T, et al. The parkinson's disease-
associated LRRK2 mutation R1441G inhibits neuronal differentiation of neural stem cells. Stem Cells Dev. 2013;22(18):2487-2496. doi:10.1089/scd.2013.
0163
(10) Winner B, Melrose HL, Zhao C, et al. Adult neurogenesis and neurite
outgrowth are impaired in LRRK2 G2019S mice. Neurobiol Dis. 2011;41(3):706-
716. doi:10.1016/j.nbd.2010.12.008