https://www.reddit.com/r/COVID19/comments/ismdn7/unusual_features_of_the_sarscov2_genome/ level 1 mushroooooooooom 2 points · 29 minutes ago Figure 8 is plain stupid from synthetic biology perspective. Even if RNA virus could be modified as easy as this, rather spend hours to do long PCR to clone the S protein and make restriction sites, just go and synthesize a few segments of the designed sequence and ligate them using Gibson assembly or overlapping PCR. This just save more time, more accurate, less labour intensive and won't leave any restriction sites.
回复 13楼stainlessbelief的帖子 你是不是看不懂英语,上面贴了论据没看到??? 要不要用下翻译?? https://www.reddit.com/r/COVID19/comments/ismdn7/unusual_features_of_the_sarscov2_genome/ level 1 mushroooooooooom 2 points 29 minutes ago Figure 8 is plain stupid from synthetic biology perspective. Even if RNA virus could be modified as easy as this, rather spend hours to do long PCR to clone the S protein and make restriction sites, just go and synthesize a few segments of the designed sequence and ligate them using Gibson assembly or overlapping PCR. This just save more time, more accurate, less labour intensive and won''t leave any restriction sites. 我已经给出了这文章贴到专业社区的讨论结果,mushroooooooooom就是个专业人士,回复看不懂??? 文贵雇的人给钱也太低了,不找几个知识水平高的来吗
https://www.reddit.com/r/COVID19/comments/ismdn7/unusual_features_of_the_sarscov2_genome/ level 1 mushroooooooooom 2 points · 29 minutes ago Figure 8 is plain stupid from synthetic biology perspective. Even if RNA virus could be modified as easy as this, rather spend hours to do long PCR to clone the S protein and make restriction sites, just go and synthesize a few segments of the designed sequence and ligate them using Gibson assembly or overlapping PCR. This just save more time, more accurate, less labour intensive and won''t leave any restriction sites.
ntkrnl 发表于 2020-09-14 13:09
这个reddit user 连PhD都不是,就是个master,GPA还极低,还想申请来美国读博呢,你说是信谁好呢? https://www.reddit.com/r/gradadmissions/comments/hwn2ji/phd_application_and_lor_advice_needed/ Hello reddit, I''m an international applicant that wants to apply for a PhD program in US to research on either microbiome or organoid research. I am not a traditional applicant and the following is my background: Undergrad: 3.2/4.0 (Biochemistry) 3.1/4.0 (Pharmacy) Research Masters 3.8/4.0 (research work is on drug discovery and teratogenicity) All degrees awarded from THE and QS top 50 worldwide university Not intended to take GRE as all my targets do not require GRE and I don''t have the time to prepare well for it Overseas licenced pharmacist 8 years of research experience, in which 6 years are in the same lab (which will lead to my LOR issue) 3 research publications (2 first author), they are Q1 and 2 in the field 1 research article to be published in a local professional journal, peer-reviewed but neither have impact factor nor indexed in pubmed 2 more manuscripts under review, in which 1 is first author 8 conferences, with 3 conference awards (2 local and 1 from US) 10 academic scholarships, in which one is full scholarship awarded for my masters degree, 3 times government scholarship recepient Currently working in a pharma company, 80% on regulatory affairs 20% on research, but the topic is on pharmacy practice Currently aiming UCSF, UCSD, Weill-Cornell, PennU, UW
回复 13楼stainlessbelief的帖子 你是不是看不懂英语,上面贴了论据没看到??? 要不要用下翻译?? https://www.reddit.com/r/COVID19/comments/ismdn7/unusual_features_of_the_sarscov2_genome/ level 1 mushroooooooooom 2 points 29 minutes ago Figure 8 is plain stupid from synthetic biology perspective. Even if RNA virus could be modified as easy as this, rather spend hours to do long PCR to clone the S protein and make restriction sites, just go and synthesize a few segments of the designed sequence and ligate them using Gibson assembly or overlapping PCR. This just save more time, more accurate, less labour intensive and won''t leave any restriction sites. 我已经给出了这文章贴到专业社区的讨论结果,mushroooooooooom就是个专业人士,回复看不懂??? 文贵雇的人给钱也太低了,不找几个知识水平高的来吗
https://www.reddit.com/r/COVID19/comments/ismdn7/unusual_features_of_the_sarscov2_genome/ level 1 mushroooooooooom 2 points · 29 minutes ago Figure 8 is plain stupid from synthetic biology perspective. Even if RNA virus could be modified as easy as this, rather spend hours to do long PCR to clone the S protein and make restriction sites, just go and synthesize a few segments of the designed sequence and ligate them using Gibson assembly or overlapping PCR. This just save more time, more accurate, less labour intensive and won't leave any restriction sites.
https://www.reddit.com/r/COVID19/comments/ismdn7/unusual_features_of_the_sarscov2_genome/ level 1 mushroooooooooom 2 points · 29 minutes ago Figure 8 is plain stupid from synthetic biology perspective. Even if RNA virus could be modified as easy as this, rather spend hours to do long PCR to clone the S protein and make restriction sites, just go and synthesize a few segments of the designed sequence and ligate them using Gibson assembly or overlapping PCR. This just save more time, more accurate, less labour intensive and won't leave any restriction sites.
她说如不向世界讲真话 她会后悔一辈子…
https://nypost.com/2020/09/11/chinese-virologist-says-she-has-proof-covid-19-was-made-in-wuhan-lab/amp/?utm_source=twitter_sitebuttons&utm_medium=site%20buttons&utm_campaign=site%20buttons&__twitter_impression=true
🔥 最新回帖
今年8月才注册的ID你自high啥啊
大批无毛举报, twitter先suspend了,不要急,很快就会恢复的
闫女士是不是英雄你说了不算。 既然不想参与那你就乖乖闭嘴啊,难不成身后有人拿枪指着你强迫你张嘴说话?
还有手动给你👎
我记得是没有,任何人可以DROP.
🛋️ 沙发板凳
https://zenodo.org/record/4028830#.X1-jHSQiclS
level 1 mushroooooooooom 2 points · 29 minutes ago Figure 8 is plain stupid from synthetic biology perspective. Even if RNA virus could be modified as easy as this, rather spend hours to do long PCR to clone the S protein and make restriction sites, just go and synthesize a few segments of the designed sequence and ligate them using Gibson assembly or overlapping PCR. This just save more time, more accurate, less labour intensive and won't leave any restriction sites.
先别着急戴帽子攻击,她丢人是她的事情 。
看来你是行内人懂不少,能分析分析她说的她的文章哪里是错的?
发nature了么,再不济发个biorxiv,论文审核通过了么,preprint就随便上传到网站,0 views 也敢出来宣扬么
不是吧,黑网站有用吗?能把各大期刊网站都黑了?能把期刊都给烧掉?
不管她说的是不是真的成立不成立,你的论据没有一毛钱价值,当然,你这一帖还是值两毛的。
爱因斯坦提出相对论的时候,只是个专利局审查员,不影响他的论文改变世界。闫梦丽好歹在相关实验室做过多年研究,也有高档次文章发表。
你们五毛总是用这老一套来抹黑,大家都清楚得很,她说的要不是真的,大外宣也不会放你们出来乱咬。
你是不是看不懂英语,上面贴了论据没看到??? 要不要用下翻译?? https://www.reddit.com/r/COVID19/comments/ismdn7/unusual_features_of_the_sarscov2_genome/ level 1 mushroooooooooom 2 points 29 minutes ago Figure 8 is plain stupid from synthetic biology perspective. Even if RNA virus could be modified as easy as this, rather spend hours to do long PCR to clone the S protein and make restriction sites, just go and synthesize a few segments of the designed sequence and ligate them using Gibson assembly or overlapping PCR. This just save more time, more accurate, less labour intensive and won''t leave any restriction sites.
我已经给出了这文章贴到专业社区的讨论结果,mushroooooooooom就是个专业人士,回复看不懂??? 文贵雇的人给钱也太低了,不找几个知识水平高的来吗
感情只有教授才接触科学?
发现DNA双螺旋结构的得到诺贝尔奖的科学家连个博士都不是。
这个reddit user 连PhD都不是,就是个master,GPA还极低,还想申请来美国读博呢,你说是信谁好呢?
https://www.reddit.com/r/gradadmissions/comments/hwn2ji/phd_application_and_lor_advice_needed/
Hello reddit, I''m an international applicant that wants to apply for a PhD program in US to research on either microbiome or organoid research. I am not a traditional applicant and the following is my background: Undergrad: 3.2/4.0 (Biochemistry) 3.1/4.0 (Pharmacy) Research Masters 3.8/4.0 (research work is on drug discovery and teratogenicity) All degrees awarded from THE and QS top 50 worldwide university Not intended to take GRE as all my targets do not require GRE and I don''t have the time to prepare well for it Overseas licenced pharmacist 8 years of research experience, in which 6 years are in the same lab (which will lead to my LOR issue) 3 research publications (2 first author), they are Q1 and 2 in the field 1 research article to be published in a local professional journal, peer-reviewed but neither have impact factor nor indexed in pubmed 2 more manuscripts under review, in which 1 is first author 8 conferences, with 3 conference awards (2 local and 1 from US) 10 academic scholarships, in which one is full scholarship awarded for my masters degree, 3 times government scholarship recepient Currently working in a pharma company, 80% on regulatory affairs 20% on research, but the topic is on pharmacy practice Currently aiming UCSF, UCSD, Weill-Cornell, PennU, UW
reddit 连个屁都不算吧?有的人老外说点啥都信以为真,看最后这片paper能不能过peer review才是正经。
爱因斯坦当时的教授看不上他,不给他大学里的工作。幸好瑞士政府给他个小公务员做。
黑这位女英雄的ID都是五毛。
大陆民科不要太多,很多微积分都不懂得,张嘴都是当年爱因斯坦如何如何................
都卖南瓜了,这一年过的实在太憋屈!
病毒开始在中共国,不是别的地方,中共就该死。
所以WM们在这洗没有用,全世界自有大批相关领域的人们去下载了好好研究。真的假不了,假的也真不了。当年切尔诺贝利捂那么严,到最后不什么细节都披露出来了。
这边司法本来就慢,一些官司可以搞1 年以上。波士顿马拉松那个娃今年才判。
说风凉话的基本都是居心叵测,不管民间怎样,重磅人物Rudy Guilian 已经明确指出这是生化袭击。这几周都会慢慢出来
有个问题你要清楚, 她的专业是眼科,根本不具备病毒等相关专业的知识的。
我想问下你周末发的无人机放火的帖子改了没有?
赔黑人37万的都没改,无人机放火算什么。
用造谣反共是一大发明,造谣是天赋人权
闫博士是湘雅的眼科MD和港大的病毒学PHD。
只是博士后,而且你要是行内的,一看就明白她一直在做什么。
但是造谣的结果是credit越来越低。 随便列下过去几个月华人的造谣热帖 盖茨基金会和WHO邮件被黑客公布 三峡大坝要崩溃(若干贴)... 贝鲁特大爆炸的硝酸铵是中国一带一路的货物 加州山火是华人无人机纵火 加州法案要赔偿每个黑人37万美元 ....................
你的CNN说哪件事不是造谣?还绝不辟谣的往死里造谣。金正恩死了?川普上台美国经济崩溃了。川普要被弹劾了。美国宪法没规定和平示威。你党妈要造谣扣credit早已经负1亿分了。要不来个造谣扣分比赛,你说一个川粉故意造谣的,我就给你拿10条党媒左媒造谣的还你,敢不敢
当年希特勒也是靠造谣犹太人纵火扫清敌人的 不过人家至少不是造自己民族的谣 不像您 造谣美国华人纵火 真卑鄙
华人上每个被扣了五毛外宣帽子的id,都算是你们造的一个谣,
来吧
就是年初的阴谋论改用英文写了。大笑话。这种垃圾是不会有peer reviewed 正规journal发的,这些人不要脸,journals是要脸的。所以放在zenodo这种不需要同行评议,谁都能发的地方,相当于自媒体blog性质。
闫水平太差,所以自己都不明白自己在出多大洋相。独运轮郭的拥趸低学历政庇偷渡跳船的为主,也看不懂,也不知道这些东西有多丢人现眼,到处当宝贝呈献。
笑死个人。
哈哈哈,不会有的。这个阴谋论里的“科学”成分低劣到让人瞠目结舌,没有哪个严肃的scientist会自贬身份掺合这个笑话。
这跟去年那个香港黄什么到哥大演讲一样,支持反对的都有,这本来很正常。但是反对的,支持中共的可以毫无顾虑的出现,还可以接受各大媒体采访。但支持黄的,连去听一下,都有很多顾虑,怕被党国的媒体拍到了,以后回中国有麻烦。
所以这女的,敢出来说,勇气就够了。
对的,不懂又一本正经的胡说,是需要很多勇气。
内行人是不会跟着掺和她这些的。
你如何说明你自己懂?然后别人不懂?
同意。这世界正常的人居多,是非黑白大家心中有数。但真能有勇气站出来的还是少数。
其实,学术对学术,如果厉害国也有能人站出来,用paper来逐条反驳闫博士的论点,就如战场上两员大将对挑,那我们大家就当看一场学术对峙。可惜,只看到各个中文网站WM们上蹿下跳地洗,反而落了下乘。
zan
手动点踩
华人键盘宣传CCP疫苗多么先进可靠信誓旦旦八月回国打疫苗的骗子居然也敢没事人一样骂别人是骗子,华人管理层把这论坛整治的这样0credit的整天现眼
人家当然懂,早就说八月回国打疫苗死活不忘自己身上扎心里能不懂吗?
那个谣还不一定是原创,我在推特上也看到,也不知道是谁最先放出来的。
https://forums.huaren.us/showtopic.html?topicid=2579729
去学习吧。
可能是这个团队看到我发的这个,就不再继续提“张实验室故意上传错误序列”这个暴露闫有多外行的可笑的指控了。
她的这篇”科学论文“里,还在说张永振实验室被”整肃“是因为发表序列暴露了新冠和舟山蝙蝠冠状病毒的亲缘关系。殊不知全世界随便一个差不多实验室都有能力给新冠病毒测序,随便一个生物口的都能构建进化树看到这种进化亲缘关系。这种整肃的理由的猜测有多滑稽可笑。
所谓舟山蝙蝠冠状病毒为骨架更是专业上荒唐可笑的认定,进一步暴露这个团队的无知低能。
更多的点对点的专业上的错误批驳懒得写了,多数人也看不懂,不值得浪费我的时间。
这篇所谓”科学论文“里从头到尾都是以猜测为事实,猜测之上叠加猜测,前面的猜测变成了后面猜测的事实基础。荒谬透顶。
lay people只要看正经科学界都无视就明白,这就是一场荒诞剧。
给你手动点赞。闫博士最开始出来爆料的时候我就说过她是检验五毛的试金石。果不其然,从这个帖子的回复中我已经屏蔽了好几个攻击她的人。
说川粉低智商,racist,崇拜集权,黄皮奴隶,川普facist,歧视女性,川普帕金森,恋女,恋童,3k,10个还你
哈哈哈,又穿新马甲了。
中国灭活疫苗还没有面向公众接种。我要能搞到手早就给自己注射了。你有办法能搞到,送给我好不好?
这段还挺多语法错误的,估计不是英语国家的人写的……和中文文法习惯还挺像的。
这个文章太专业了,而且有好几位作者,估计99.9%以上的人看不懂,让科学界去讨论吧!
有很多方法达到改变病毒基因的目的。这不是要点。 要点是病毒是人工的
你又算什么玩意?
你先出点证据证明你有资格看论文。科学论文什么时候变成阿狗阿猫都可以出来随便解读了。我先承认我是看不懂的。
先说说你审过什么期刊的稿,这样也不会暴露身份。
就像当年张益唐的论文出来,一大堆mitbbs 说论证有误,还说得像模像样的。
你要是有过理科博士的训练,看不懂专业名词也能看明白那篇东西符合不符合scientific method。里面是opinions, wild claims的堆砌还是fact-based reasoning 还是能看明白的。
我的资格不需要向你证明,接受不接受我的观点我也不care。
是不是垃圾阴谋论,科学界的无视就说明了一切。
不信就继续等着呗。
李文亮出来爆料的时候,一堆五毛攻击人家是眼科医生。
要新时代的武昌起义!
为什么这年头还有人拿法币发帖的?法币几十年前就不值钱了,大法都做不到与时俱进啊
不早就跟你说上微博发帖求扎针大妈送你上热搜党妈正好缺典型你肯定扎上嘛……装傻充愣好几个月了死活不扎是怎么回事?难怪是专家呀……心里明镜似的
“基因组序列就像人类指纹,您可以基于此识别它们。我将使用证据告诉您为何病毒来自武汉实验室。即使您没有生物学知识,也可以阅读并可以检查,识别和验证”。所以如果阿猫阿狗不能正确解读的话,只能说闫博士没有把这个report写成当初她说的那样。
只敢穿马甲的赶紧闭嘴吧
确实是,如果有实证,估计能发全球顶级期刊,一战成名。还等什么?
是不是实证和发顶级期刊根本没有什么关系,让大家都看看有什么不好
大家都在研究论文的正确性,你却拿个所谓的经济学家出来。 暴露智商了吧。
你所谓的“重磅”人物是指体重吧,没有生化专家来论证,律师,经济顾问跳出来叫,还真有人会信。这都什么智商啊。
这贴文贵雇来的那些id(可能是同一个人?)怎么不来读读论文,分析分析,不是说来讨论的吗,为啥就发个链接,然后拼命说这个就是正确的,然后就跑了,文贵雇的人文化水平这么低么
屠呦呦是正经教授吗?