作者贡献： 第一作者兼并列通讯作者： V.D.M. designed, coordinated and performed experiments, completed analysis and wrote the manuscript. 通讯作者：R.S.B. designed experiments and wrote manuscript.
AUTHOR CONTRIBUTIONSV.D.M. designed, coordinated and performed experiments, completed analysis andwrote the manuscript. B.L.Y. designed the infectious clone and recovered chimericviruses; S.A. completed neutralization assays; L.E.G. helped perform mouseexperiments; T.S. and J.A.P. completed mouse experiments and plaque assays;X.-Y.G. performed pseudotyping experiments; K.D. generated structural figuresand predictions; E.F.D. generated phylogenetic analysis; R.L.G. completed RNAanalysis; S.H.R. provided primary HAE cultures; A.L. and W.A.M. provided criticalmonoclonal antibody reagents; and Z.-L.S. provided SHC014 spike sequences andplasmids. R.S.B. designed experiments and wrote manuscript. Xing-Yi Ge
researcher at the University of North Carolina at Chapel Hill, last week (November 9) published a study on his team’s efforts to engineer a virus with the surface protein of the SHC014 coronavirus, found in horseshoe bats in China, and the backbone of one that causes human-like severe acute respiratory syndrome (SARS) in mice. The hybrid virus could infect human airway cells and caused disease in mice, according to the team’s results, which were published in Nature Medicine.
算了吧 tg自己的锅 已经证实过多起由于tg疫苗设计不当，引起新的epidemic Live-attenuated vaccines remain key players in reducing the global disease burden associated with viral infections in humans, critically important livestock, and companion animals. Historically and contemporarily, live-attenuated vaccines have been used with success to help control measles, mumps, rubella, polio, yellow fever, and chickenpox infections and outbreaks1,21,22. However, live-attenuated vaccines are also associated with the risk of reversion by either mutation- or recombination-driven processes, which can cause dangerous outbreaks in unvaccinated populations, including animals22. For example, highly pathogenic porcine epidemic diarrhea virus (PEDV) strains emerged in China in 2012, circumventing existing vaccines, and RNA recombination events between wild-type and live-attenuated PEDV and between avian infectious bronchitis virus (IBV) strains have seeded new outbreaks23,24,25,26. Therefore, measures are needed to stabilize live-attenuated vaccines against reversion under selective pressure, particularly for viruses like CoVs, which employ recombination as a standard feature of their replication cycle, as incidental recombination events in the context of a co-infection could unintentionally introduce alleles with enhanced virulence into an attenuated vaccine genome, with consequences that could be difficult to predict13,27,28. Several groups, including our own, have developed novel strategies in fidelity regulation and control that attenuate RNA virus pathogenesis, and in the case of CoVs, the development of mutants that prevent reversion repair to virulence1,29.
AUTHOR CONTRIBUTIONSV.D.M. designed, coordinated and performed experiments, completed analysis andwrote the manuscript. B.L.Y. designed the infectious clone and recovered chimericviruses; S.A. completed neutralization assays; L.E.G. helped perform mouseexperiments; T.S. and J.A.P. completed mouse experiments and plaque assays;X.-Y.G. performed pseudotyping experiments; K.D. generated structural figuresand predictions; E.F.D. generated phylogenetic analysis; R.L.G. completed RNAanalysis; S.H.R. provided primary HAE cultures; A.L. and W.A.M. provided criticalmonoclonal antibody reagents; and Z.-L.S. provided SHC014 spike sequences andplasmids. R.S.B. designed experiments and wrote manuscript. Xing-Yi Ge
-- 发信人: Dale08 (小鼠鼠), 信区: Military 标 题: Re: 美国人把蝙蝠身上的冠状病毒改变成容易感染人 发信站: BBS 未名空间站 (Tue Feb 4 13:50:00 2020, 美东)
作者贡献： 第一作者兼并列通讯作者： V.D.M. designed, coordinated and performed experiments, completed analysis and wrote the manuscript. 通讯作者：R.S.B. designed experiments and wrote manuscript.
如果真是这个病毒出问题， 主要负责人是美国人。
下面是引述
--
发信人: Dale08 (小鼠鼠), 信区: Military
标 题: Re: 美国人把蝙蝠身上的冠状病毒改变成容易感染人
发信站: BBS 未名空间站 (Tue Feb 4 13:50:00 2020, 美东)
作者贡献：
第一作者兼并列通讯作者： V.D.M. designed, coordinated and performed
experiments, completed analysis and wrote the manuscript.
通讯作者：R.S.B. designed experiments and wrote manuscript.
中国石研究员贡献：Z.-L.S. provided SHC014 spike sequences and plasmids.
这个表达质粒来源于石研究院组2013已经发表的Nature 论文。request已经发表文章里
的一般性实验材料，石研究员本身就有义务提供给同行。这个authorship是礼节性的，
挂名，更常见的处理是放在致谢栏目里。
这么清晰明了的事实，华人网和买买提一帮轮子老将反反复复拿来做攻击石正丽的材料
，给石扣合成人工病毒的帽子，你他妈的是要一遍一遍告诉大家自己是弱智文盲大傻逼么
---
AUTHOR CONTRIBUTIONSV.D.M. designed, coordinated and performed experiments, completed analysis andwrote the manuscript. B.L.Y. designed the infectious clone and recovered chimericviruses; S.A. completed neutralization assays; L.E.G. helped perform mouseexperiments; T.S. and J.A.P. completed mouse experiments and plaque assays;X.-Y.G. performed pseudotyping experiments; K.D. generated structural figuresand predictions; E.F.D. generated phylogenetic analysis; R.L.G. completed RNAanalysis; S.H.R. provided primary HAE cultures; A.L. and W.A.M. provided criticalmonoclonal antibody reagents; and Z.-L.S. provided SHC014 spike sequences andplasmids. R.S.B. designed experiments and wrote manuscript.
Xing-Yi Ge
----------------------------------
https://www.the-scientist.com/news-opinion/lab-made-coronavirus-triggers-debate-34502
我不是持美国阴谋论啊，但这种研究似乎很普遍
Lab-Made Coronavirus Triggers DebateThe creation of a chimeric SARS-like virus has scientists discussing the risks of gain-of-function research.Nov 16, 2015JEF AKST
[url=][/url][url=][/url][url=][/url][url=][/url]22.5K
Ralph Baric, an infectious-disease
researcher at the University of North Carolina at Chapel Hill, last week (November 9) published a study on his team’s efforts to engineer a virus with the surface protein of the SHC014 coronavirus, found in horseshoe bats in China, and the backbone of one that causes human-like severe acute respiratory syndrome (SARS) in mice. The hybrid virus could infect human airway cells and caused disease in mice, according to the team’s results, which were published in Nature Medicine.
---
lz是这个表情
解放军防化部队早进武汉了
如果真是这个病毒出问题， 主要负责人是美国人。
下面是引述
---
AUTHOR CONTRIBUTIONSV.D.M. designed, coordinated and performed experiments, completed analysis andwrote the manuscript. B.L.Y. designed the infectious clone and recovered chimericviruses; S.A. completed neutralization assays; L.E.G. helped perform mouseexperiments; T.S. and J.A.P. completed mouse experiments and plaque assays;X.-Y.G. performed pseudotyping experiments; K.D. generated structural figuresand predictions; E.F.D. generated phylogenetic analysis; R.L.G. completed RNAanalysis; S.H.R. provided primary HAE cultures; A.L. and W.A.M. provided criticalmonoclonal antibody reagents; and Z.-L.S. provided SHC014 spike sequences andplasmids. R.S.B. designed experiments and wrote manuscript.
Xing-Yi Ge
--
发信人: Dale08 (小鼠鼠), 信区: Military
标 题: Re: 美国人把蝙蝠身上的冠状病毒改变成容易感染人
发信站: BBS 未名空间站 (Tue Feb 4 13:50:00 2020, 美东)
作者贡献：
第一作者兼并列通讯作者： V.D.M. designed, coordinated and performed
experiments, completed analysis and wrote the manuscript.
通讯作者：R.S.B. designed experiments and wrote manuscript.
中国石研究员贡献：Z.-L.S. provided SHC014 spike sequences and plasmids.
这个表达质粒来源于石研究院组2013已经发表的Nature 论文。request已经发表文章里
的一般性实验材料，石研究员本身就有义务提供给同行。这个authorship是礼节性的，
挂名，更常见的处理是放在致谢栏目里。
这么清晰明了的事实，华人网和买买提一帮轮子老将反反复复拿来做攻击石正丽的材料
，给石扣合成人工病毒的帽子，你他妈的是要一遍一遍告诉大家自己是弱智文盲大傻逼么