http://virological.org/t/initial-genome-release-of-novel-coronavirus/319 Initial genome release of novel coronavirus Novel 2019 coronavirus edward_holmes 3 1d 10th January 2020 This posting is communicated by Edward C. Holmes, University of Sydney on behalf of the consortium led by Professor Yong-Zhen Zhang, Fudan University, Shanghai The Shanghai Public Health Clinical Center & School of Public Health, in collaboration with the Central Hospital of Wuhan, Huazhong University of Science and Technology, the Wuhan Center for Disease Control and Prevention, the National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control, and the University of Sydney, Sydney, Australia is releasing a coronavirus genome from a case of a respiratory disease from the Wuhan outbreak. The sequence has also been deposited on GenBank (accession MN908947) and will be released as soon as possible. The sequence can be downloaded here: WH-Human_1.fasta.gz (8.9 KB – this is a fasta file compressed using gzip. Uncompress using gzip -d WH-Human_1.fasta.gz) Disclaimer: Please feel free to download, share, use, and analyze this data. We ask that you communicate with us if you wish to publish results that use these data in a journal. If you have any other questions –then please also contact us directly. Professor Yong-Zhen Zhang, Shanghai Public Health Clinical Center & School of Public Health, Fudan University, Shanghai, China. email: [email][email protected][/email] Initial genome release of novel coronavirus Novel 2019 coronavirus
刚看到这个： “Regarding early prospects for testing, Menachery noted that the nucleocapsid protein for coronaviruses is highly conserved across coronavirus families, and the N protein of nCoV is about 90% conserved with the SARS N protein. Though less than some other coronaviruses, the similarity may signal nCoV cross-reactivity with the SARS N protein. If there is cross-reactivity, commercially available SARS N protein might allow screening of patient sera.” http://www.cidrap.umn.edu/news-perspective/2020/01/china-releases-genetic-data-new-coronavirus-now-deadly 大意就是冠状病毒的N蛋白是高度保守的，这种新型病毒的N蛋白和SARS的N蛋白有90%相似。有些冠状病毒的N蛋白与SARS有更高的相似度，不过这种新病毒的N蛋白和SARS的N蛋白仍可能有cross-reactivity。如果是这样，现在的商用SARS N蛋白可以用来检测血清中这种新病毒的N蛋白是否存在。
可能性基本不存在。 http://www.cidrap.umn.edu/news-perspective/2020/01/china-releases-genetic-data-new-coronavirus-now-deadly No sign of human-to-human spread Based on the current reports, there's no obvious evidence of human-to-human spread yet, Osterholm said, noting that red flags, for example, would be infections in patients' family members who weren't at the market and infections in healthcare workers. The outbreak update posted last night by Wuhan's health commission was its first since Jan 5. The report was quickly translated and posted by FluTrackers, an infectious disease news message board, and the details also appeared in an English language statement from Hong Kong's Centre for Health Protection (CHP). Wuhan officials said 41 patients have been diagnosed with nCoV pneumonia, and 2 have been discharged from the hospital. Seven had severe infections, and 1 patient died. The rest are listed in stable condition. The patient who died is apparently a 61-year-old man who had chronic liver disease and was a frequent customer at the market at the center of the investigation, according to a translation of a Chinese media report posted on Twitter by Hayes Luk, PhD, a microbiologist at the University of Hong Kong. So far 739 close contacts have been identified for monitoring, 419 of them medical staff. No related cases have been detected. Investigators haven't found any clear evidence of human-to-human spread, and no infections have been found in healthcare workers. Most of the patients were workers at the seafood market at the center of the outbreak, which also sold live animals and meat from wildlife. The most recent illness was reported on Jan 3.
可能性基本不存在。 http://www.cidrap.umn.edu/news-perspective/2020/01/china-releases-genetic-data-new-coronavirus-now-deadly No sign of human-to-human spread Based on the current reports, there's no obvious evidence of human-to-human spread yet, Osterholm said, noting that red flags, for example, would be infections in patients' family members who weren't at the market and infections in healthcare workers. The outbreak update posted last night by Wuhan's health commission was its first since Jan 5. The report was quickly translated and posted by FluTrackers, an infectious disease news message board, and the details also appeared in an English language statement from Hong Kong's Centre for Health Protection (CHP). Wuhan officials said 41 patients have been diagnosed with nCoV pneumonia, and 2 have been discharged from the hospital. Seven had severe infections, and 1 patient died. The rest are listed in stable condition. The patient who died is apparently a 61-year-old man who had chronic liver disease and was a frequent customer at the market at the center of the investigation, according to a translation of a Chinese media report posted on Twitter by Hayes Luk, PhD, a microbiologist at the University of Hong Kong. So far 739 close contacts have been identified for monitoring, 419 of them medical staff. No related cases have been detected. Investigators haven't found any clear evidence of human-to-human spread, and no infections have been found in healthcare workers. Most of the patients were workers at the seafood market at the center of the outbreak, which also sold live animals and meat from wildlife. The most recent illness was reported on Jan 3.
Flu vaccines are updated to better match viruses expected to be circulating in the United States. The A(H1N1)pdm09 vaccine component was updated from an A/Michigan/45/2015 (H1N1)pdm09-like virus to an A/Brisbane/02/2018 (H1N1)pdm09-like virus. The A(H3N2) vaccine component was updated from an A/Singapore/INFIMH-16-0019/2016 A(H3N2)-like virus to an A/Kansas/14/2017 (H3N2)-like virus. Both B/Victoria and B/Yamagata virus components from the 2018-2019 flu vaccine remain the same for the 2019-2020 flu vaccine.
刚看到这个： “Regarding early prospects for testing, Menachery noted that the nucleocapsid protein for coronaviruses is highly conserved across coronavirus families, and the N protein of nCoV is about 90% conserved with the SARS N protein. Though less than some other coronaviruses, the similarity may signal nCoV cross-reactivity with the SARS N protein. If there is cross-reactivity, commercially available SARS N protein might allow screening of patient sera.” http://www.cidrap.umn.edu/news-perspective/2020/01/china-releases-genetic-data-new-coronavirus-now-deadly 大意就是冠状病毒的N蛋白是高度保守的，这种新型病毒的N蛋白和SARS的N蛋白有90%相似。有些冠状病毒的N蛋白与SARS有更高的相似度，不过这种新病毒的N蛋白和SARS的N蛋白仍可能有cross-reactivity。如果是这样，现在的商用SARS N蛋白可以用来检测血清中这种新病毒的N蛋白是否存在。
另外，之前我还猜测中科院病毒所会研究这个病毒，昨天发布的基因组数据是由复旦和澳大利亚合作提供的，猜错了。>_< ostrakon 发表于 1/12/2020 12:11:24 AM
“这次武汉重症肺炎的病毒分析出来了，是冠状病毒的变异，就是03年SARS病毒的变异种，毒性很高，北京的各大医院都被要求开始严防死守发热患者了！你们出门一定带口罩”！！！
🔥 最新回帖
🛋️ 沙发板凳
感冒病毒都是冠状病毒，都是sars亲戚，这个放医说了等于没说。
新的冠状病毒是确认 只是说没发生人传染人
以下为 Science 报道的全文翻译。生态健康联盟总裁Peter Daszak表示：“中国的病毒学家是世界上最优秀的病毒学家，他们的工作速度非常快，效率极高，他们掌握的信息比我们现在知道的要多得多，这次疫情是中国展示他们在21世纪的公共卫生和病毒学方面所做的努力的一个机会。”虽然致病原和疾病之间的联系还有待证实，但许多科学家对这一发现表示称赞，他们说这是中国在病毒学方面实力的证明。但是他们敦促中国尽快分享更多关于这种新病原体的信息，包括该新病原体的序列信息、可能引起的疾病以及传播途径。伊拉斯姆斯医学中心的病毒学家Marion Koopmans表示：“我认为他们真的应该共享序列数据，这样我们就能确保如果我们有来自这个地区的旅客，我们就能检测出这种病毒。”另外，新华社今天确认了此次疫情调查的负责人是徐建国。尽管该机构没有透露他的具体工作单位，徐建国显然是在中国疾病预防控制中心的中国传染病预防控制所工作。徐建国告诉新华社，研究人员正在继续他们的工作，以确认冠状病毒是罪魁祸首。世界卫生组织（WHO）驻中国代表Gauden Galea今天在一份新闻声明中写道：“在短时间内初步鉴定出一种新病毒是一项显著的成就，表明中国在处理新疫情方面的能力有所提高。”世界各地的科学家都认同这种观点，但他们还想了解更多。香港大学病毒学家Malik Peiris表示，“应该祝贺中国研究人员迅速鉴定出病原体。现在他们与世卫组织和全球公共卫生界共享该新型病毒特异的诊断性RT-PCR 测试至关重要。”生态健康联盟总裁Peter Daszak说：“我真正想看到的是有关流行病学和病理学的信息，这样我们就都可以确信：第一，这种冠状病毒是这次爆发的原因；第二，它被控制住了，他们已经能够追踪所有的潜在病例，以便进行隔离和检测。在我看来，如果我们得不到全部的信息，疫情就有进一步传播的风险。”德国柏林大学Charite医院的Christian Drosten说：“中国的研究人员不必担心共享信息会妨碍这种新病毒在权威杂志上发表，没有任何杂志会因为这一序列被公开而拒绝发表其论文。”新闻报道一直谨慎地称调查结果是初步的。在2003年非典(SARS)爆发期间，中国当局和科学家感到尴尬的是过早地报道了衣原体是罪魁祸首，后来证明是一种新的冠状病毒。杜克-新加坡国立大学医学院新兴疾病专家Wang Linfa说：“我理解为什么政治家和科学家在宣布这种新型病毒时必须格外谨慎。确定这种联系的关键步骤是在实验动物身上重现这些症状，但这可能需要几周或几个月的时间。”Wang Linfa说，武汉爆发的疫情和SARS之间的相似之处很有趣。这两起病例都出现在冬季，最初的病例与接触活禽市场出售的动物有关。(在非典疫情中，中间宿主被证明是市场上出售的果子狸。)
在香港，最近几周去武汉旅行的48人中，有人出现了发烧，呼吸道感染或肺炎的症状。新加坡和韩国也隔离了来自武汉的患病旅客。到目前为止，这些人中尚未发现有人被感染疑似病毒。
原文链接：https://www.sciencemag.org/news/2020/01/scientists-urge-china-quickly-share-data-virus-linked-pneumonia-outbreak本文由BioWorld公众号原创编译，如需转载，请留言或添加管理员微信：bwzhushou
基因相似度80% 还有洗地的那简直是丧心病狂了. 不明白情况通报像香港一样的随时更新情况引起大家重视有什么不对？!
人命关天即使过度紧张了又不会死人，忽略或者故意粉饰太平才是害人
哪儿有靠得住的信息？
你和黑猩猩genome 相似度99%。 所以我们可以叫你黑猩猩？
80% 还真的不算啥
外行就不要胡言乱语耸人听闻造谣了。
什么SARS变异来的。笑死。
一定要想办法抹黑，一次次丢人现眼。
不明白整天抹黑抹黑阴谋论要说明什么? 抹黑什么？ 抹黑病毒?
重视它 空开信息有什么错？ 重视它会死人吗 ? 一定要等事情到不可收拾再重视？
人和人基因还百分百相似呢，但不是东西就永远不是东西。
你可以选不重视 但是无权让别人也不重视。信息公布让大家去重视这件事情有什么错？
ls 99%抬杠不算啥的都说出口来 80% 的确不算啥
百分之几只说明该重视了 不是说是什么。
是不是能感染人，最重要的是病毒表面能和人上皮细胞受体结合的尖刺蛋白。SARS就是野生冠状病毒突变，造成表面尖刺蛋白能和人类细胞表面受体结合，获得进入人类细胞内的能力，所以可以从动物跳到人，感染人类。
然后SARS病毒的某些特性能让人类有非常激烈的免疫反应，就造成病人发生cytokine storm, 非常容易进入休克状态，所以有高达10%致死率。
新的冠状病毒和SARS 80%相似性不重要，重要的是不是和SARS有类似的能和人类受体结合好的表面蛋白。这个是决定新病毒的致病性的关键。
变异要是在关键的地方，不要20%的不同，不到1%的不同都能造成感染能力，致病能力的巨大差异。
所以，单看80%同源性无法判断病毒对人类的感染能力和致病性，要看临床表现。
谢谢科普！
哪个病毒学家告诉你新病毒是SARS的变种了？明白病毒的新种是怎么定义的么？屁都不懂就知道耸人听闻。
你接着洗地。不过这个例子举的好，生物界只有人类和黑猩猩会对同类进行种族灭绝。
洗地幸苦哦。解释解释为什么当局不公布病毒详细数据呢？
香港袁教授的数据不是中国内地给的？他开天眼看到的？
从12月12号第一个海鲜市场病人发病，到12月30日多例海鲜市场病人发病，证明有个out break， 开始找病原体到现在也就两周不到，你要还多快。
赞科普
冬季是上呼吸道感染高发期，流感季节，肺炎高发太寻常。
这是赤脚医生么？看症状就能确诊新型冠状病毒感染。病毒性肺炎症状基本都是一样的，不做病原检测，根本无法知道。
其实医生处置病毒性肺炎都是对症和支持治疗，一般不会去查病原体的，因为治疗手段无差异，查了也没帮助，绝大多数病毒是没有有针对性的药物的。
这些微信消息来源都是没有专业知识的病人家属，除了制造恐慌屁用没有。
器官衰竭上呼吸机就是休克中晚期了，必须ICU了。在全世界医院，ICU都是紧张资源，不是要进就能进得去。
谢谢！
另外是99% identical with chimpanzees.
我看到 下面这段报道似乎香港对所有来自武汉的可疑病人（约60人）都进行了基因测试，结果都排除了新型冠状病毒的可能性。你说中国一般都不会对病人去查病原体，那会不会有可能病人死了都不知道是否死于这种新病毒呢？前几天这个论坛有个楼主发帖说河南的表姐因肺炎去医院不到一天就走了，有没有可能也是死于武汉这个或其它未知病毒？
袁國勇指，本港使用的基因測試對新型冠狀病毒有效，現時本港所有懷疑個案結果呈陰性反應。
“Regarding early prospects for testing, Menachery noted that the nucleocapsid protein for coronaviruses is highly conserved across coronavirus families, and the N protein of nCoV is about 90% conserved with the SARS N protein. Though less than some other coronaviruses, the similarity may signal nCoV cross-reactivity with the SARS N protein. If there is cross-reactivity, commercially available SARS N protein might allow screening of patient sera.”
http://www.cidrap.umn.edu/news-perspective/2020/01/china-releases-genetic-data-new-coronavirus-now-deadly
大意就是冠状病毒的N蛋白是高度保守的，这种新型病毒的N蛋白和SARS的N蛋白有90%相似。有些冠状病毒的N蛋白与SARS有更高的相似度，不过这种新病毒的N蛋白和SARS的N蛋白仍可能有cross-reactivity。如果是这样，现在的商用SARS N蛋白可以用来检测血清中这种新病毒的N蛋白是否存在。
另外，之前我还猜测中科院病毒所会研究这个病毒，昨天发布的基因组数据是由复旦和澳大利亚合作提供的，猜错了。>_<
对于轮子来说，那简直是一定确定以及肯定。绝对是！
中共掩盖了成千上百万例新SARS死亡案例。
明天就会有轮媒这么讲。
前几天那个流感楼也有好几个mms周围的人因流感急速去世，河南那个发病如果超过一天，肺部早已感染，如何证明它是武汉这种病毒？
我不关心轮子怎么认为或轮媒怎么说，你如果是轮子算我没问。你如果不是轮子我有兴趣知道你觉得有没有可能，可能性多大。
我就不明白了 大家互相奔走相告提高警惕 有什么错 ， 你想证明别人说的不准确 但能证明你的正确吗? 你能确认不会再恶化 可扩散？ 小心点有什么错 有什么错 要扯到什么轮子去
信息公开有什么问题。 你解释下为什么不公开信息 大家怕生病是要推翻共产党吗？ 至于吗。。张嘴就戴帽子有病啊这是
真的很烦上纲上线的。你觉得没事你就去武汉 ，觉得还是稳妥点的就不要去好了。命自己的，小心点没损失，但不要帮别人做主你没有资格。
可能性基本不存在。
http://www.cidrap.umn.edu/news-perspective/2020/01/china-releases-genetic-data-new-coronavirus-now-deadly
No sign of human-to-human spread Based on the current reports, there's no obvious evidence of human-to-human spread yet, Osterholm said, noting that red flags, for example, would be infections in patients' family members who weren't at the market and infections in healthcare workers. The outbreak update posted last night by Wuhan's health commission was its first since Jan 5. The report was quickly translated and posted by FluTrackers, an infectious disease news message board, and the details also appeared in an English language statement from Hong Kong's Centre for Health Protection (CHP). Wuhan officials said 41 patients have been diagnosed with nCoV pneumonia, and 2 have been discharged from the hospital. Seven had severe infections, and 1 patient died. The rest are listed in stable condition. The patient who died is apparently a 61-year-old man who had chronic liver disease and was a frequent customer at the market at the center of the investigation, according to a translation of a Chinese media report posted on Twitter by Hayes Luk, PhD, a microbiologist at the University of Hong Kong. So far 739 close contacts have been identified for monitoring, 419 of them medical staff. No related cases have been detected. Investigators haven't found any clear evidence of human-to-human spread, and no infections have been found in healthcare workers. Most of the patients were workers at the seafood market at the center of the outbreak, which also sold live animals and meat from wildlife. The most recent illness was reported on Jan 3.
原谅我小白，如果肺部已经感染就不能测出哪种病毒了吗？
信息有不公开么？
可以测啊。不过对治疗无意义。
谢谢。所以假设这个病毒没有变异，目前来说在国内的人只要不直接接触带有这种病毒的动物就不太可能有感染的危险。
是的。
估计国内会进一步确定什么动物是新冠状病毒来源。
中东的MERS 冠状病毒是从骆驼传人的。
对这个病人的治疗没意义但对病毒的数据收集有意义从而对将来的病毒防治和医治会有益处
奔走相告不是造谣传谣。
看看你的题目。
我不是学医的，病毒的知识早就还给老师了。也许吧，但是那位去世当时也没人处理收集样品，sars那篇文章里面是收集了去世病人的肺组织标本或者其他病人的鼻拭子标本。我就是说没法证明是流感还是其它情况。
前两三个月吧，走在路上有个白人女人，应该是东欧或俄罗斯的，突然喊Swine fever is coming! 我看看旁边，就我和我姐亚裔，比她喊得还大声：Yeah, Swine fever is coming, coming from Russia. 那货脸都气歪了，哈哈！
人和黑猩猩的构成 vs 病毒和病毒 的构成 能同比吗？傻逼
80%类似的话，那今年的flu shot大概率是不会让我们对这个变种病毒产生抵抗力的
不过同意洗地同学说的，致不致死，光看80%很难说吧
这种动物和人之间来回跳的总是蛮吓人的
dna比rna稳定不知道多少倍
病毒之所以大多数是rna-based就是因为变异快这个优势
即使100%matchSARS病毒打flu shot也没用，目前美国的流感预防针只包括这4种病毒：
Flu vaccines are updated to better match viruses expected to be circulating in the United States.
The A(H1N1)pdm09 vaccine component was updated from an A/Michigan/45/2015 (H1N1)pdm09-like virus to an A/Brisbane/02/2018 (H1N1)pdm09-like virus.
The A(H3N2) vaccine component was updated from an A/Singapore/INFIMH-16-0019/2016 A(H3N2)-like virus to an A/Kansas/14/2017 (H3N2)-like virus.
Both B/Victoria and B/Yamagata virus components from the 2018-2019 flu vaccine remain the same for the 2019-2020 flu vaccine.
赞专业。这是不是按理来说拿到测序结果的科研人员已经对变异有无分布在结合人类细胞的区域有大概了解，但下一步还是要做实验验证？
今天拿到基因排序了，估计两三天就能知道怀疑个案到底是不是这个病了。
bless
武汉病毒所衰败如此，本来分离个病毒对他们小事一桩，再测个序，头筹拔的妥妥的。
anyway，基本就是这个样子，新病毒和老SARS挺像，所以一开始用检测结果是SARS，就大家恐慌了一阵，然后其他方法检测不出来是老SARS，所以说排除了SARS，然后大家就怀疑掩盖真相又恐慌了一阵。然后就努力找出来到底是啥，大家都蒙在鼓里继续high。
然而，即使是RNA病毒，20%的突变也是挺罕见的。这个序列到底拼的对不对？搞清楚这个还是要时间的。
又，
放医是防疫，现在国内大家拼高大上科研，防疫方面没赶上，还衰落了。。。没人做这种不来课题不来钱的事。
这个说得实在。
有些人怕官位不保
上去人多的地方吧.
哎
我也得在武汉转车呢!
赞科普。这位确实了解病毒。
我只关注了香港的病例情况。目前为止他们把从武汉入境后有感冒咳嗽症状的人都算作疑似病例隔离起来。六十多例测下来目前还没有发现一例是武汉新病毒。我的感觉香港是很小心，宁肯把所有可疑人士都隔离测试排查了也不让任何人有漏网的可能。从香港的排查结果来看，只要没去过武汉那个海鲜市场感染新病毒的机会很小。武汉那个市场1月1日就关了，所以周边没发现新病例是可能的。